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    <ns1:title language="sr">Karakterizacija suvih ekstrakata cvasti smilja, Helichrysum plicatum DC. i ispitivanje njihove antioksidativne, citotoksične, spazmolitičke i antimikrobne aktivnosti </ns1:title>
    <ns2:subtitle language="sr">doktorska disertacija</ns2:subtitle>
    <ns2:alt_title language="en">Characterization of dry extracts of everlasting inflorescences, Helichrysum plicatum DC., and assaying their antioxidant, cytotoxic, spasmolytic and antimicrobial activity : doctoral dissertation</ns2:alt_title>
    <ns1:language>sr</ns1:language>
    <ns1:description language="sr">U ovoj doktorskoj disertaciji izvršena je karakterizacija suvih ekstrakata cvasti
smilja, Helichrysum plicatum DC. i ispitana njihova antioksidativna, citotoksična,
spazmolitička i antimikrobna aktivnost. Posebna pažnja posvećena je pronalaženju
korelacija između hemijskog sastava i farmakoloških aktivnosti suvih ekstrakata u cilju
utvrđivanja mogućih aktivnih supstanci ili aktivnih markera.
U okviru preformulacionih ispitivanja, biljna droga cvasti smilja - Helichrysi
flos (biološki izvor Helichrysum plicatum DC.) je precizno definisana, identifikovana i
ispitana. Suvi ekstrakti dobijeni su ekstrakcijom usitnjene biljne droge, primenom
metode trostruke perkolacije, prečišćavanjem dobijenog tečnog ekstrakta metodom reekstrakcije
i uparavanjem pod vakuumom. U toku procesa ekstrakcije varirana su tri
faktora: stepen usitnjenosti biljne droge, vrsta ekstragensa (40%, 50%, 60% etanol) i
vrsta rastvarača za re-ekstrakciju (5:5, 9:1, 100:0 etilacetat:etanol; v:v), na tri nivoa.
Razvijena je HPTLC fingerprintna metoda za brzu i pouzdanu identifikaciju
glikozida i aglikona flavonoida, prisutnih u biljnoj drogi i ekstraktima. Njihova
identifikacija je bazirana na različito obojenim fluorescentnim mrljama uz primenu
standarda.
Tentativna LC-UV-MS analiza ekstrakata dobijenih re-ekstrakcijom je pokazala
prisustvo 18 jedinjenja. Identifikovane su fenolne kiseline i flavonoidi. Flavonoidi su
bili zastupljeni sa 4 grupe jedinjenja: flavanoni (naringenin i njegovi derivati), flavoni
(apigenin, derivati apigenina i luteolina), flavonoli (kvercetin i kempferol i njihovi
derivati) i halkoni (izosalipurpozid). Tentativna LC-UV-MS analiza biljne droge i
ekstrakta cvasti smilja pokazala je da ima razlike u njihovim hromatogramima, zato što
su pojedine komponente u biljnoj drogi zastupljene u nižim koncentracijama i detektuju
se tek nakon procesa prečišćavanja ekstrakta. Takođe, identifikovana je komponenta
cinarin, koja do sada nije identifikovana ni u jednoj Helichrysum vrsti. Dobijeni hemijski profil H. plicatum DC. ukazuje na veliku sličnost sa oficinalnom vrstom H.
arenarium (L.) Moench, a u isto vreme i na razlike u odnosu na nju...</ns1:description>
    <ns1:description language="en">In this doctoral dissertation, the characterization of dry extracts of everlasting
inflorescences, Helichrysum plicatum DC., and assay of their antioxidant, cytotoxic,
spasmolytic and antimicrobial activity were carried out. Special attention was paid to
investigation of correlation between chemical composition and pharmacological effects
of dry extracts to determine potentially active substances or active markers.
In the preformulation study, the herbal drug Helichrysi flos (Helichrysum
plicatum DC.) was precisely defined, identified and tested. The dry extracts were
prepared from powdered herbal drug by triple percolation method and purification of
obtained liquid extracts by means of re-extraction method and vacuum evaporation. The
following factors varied in the extraction procedures at three levels: degree of fineness
of herbal drug, type of extragens (40%, 50%, 60% ethanol/water ratio) and re-extraction
solvent (5:5, 9:1, 100:0 ethilacetate/ethanol ratio; v/v).
The HPTLC fingerprint method was developed for rapid and reliable
identification of glycosides and aglicone flavonoids present in the herbal drugs and
extracts H.plicatum. This identification was based on the color fluorescence, and
application of the standards.
The tentative LC-UV-MS analysis of purified extracts revealed the presence of
18 compounds. Phenolic acids and flavonoids were also identified. As for flavonoids,
there were four groups of compounds present: flavanones (naringenin and derivates),
flavones (apigenin and derivates, luteolin), flavonols (quercetin and derivates;
kaempeferol and derivates) and halcones (isosalipurpozide). The tentative LC-UV-MS
analysis of drug and extract of everlasting inflorescences showed the difference between
chromatograms of herbal drugs and extracts, because some compounds in herbal drug
were present in small amounts and their presence was detected after extract purification
process. A cynarin component was also identified that has not been identified in any of
Helichrysum species. Thus obtained chemical profile H. plicatum DC. indicates a significant similarity to H. arenarium (L.) Moench, and at the same time the differences
present.</ns1:description>
    <ns1:description language="sr">Farmacija - Farmaceutska tehnologija, farmakognozija / Pharmacy - Pharmaceutical technology, Pharmacognosy 
Datum odbrane : 28.09.2013</ns1:description>
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    <ns7:keyword language="sr" seq="0">smilje, Helichrysum plicatum, optimizacija procesa ekstrakcije, fenolnajedinjenja, flavonoidi, antioksidativna aktivnost, antimikrobna aktivnost, citotoksičnaaktivnost, spazmolitička aktivnost</ns7:keyword>
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