o:39512 Identification and functional characterization of tumor-suppressive microRNAs with potential key roles in breast cancer progression and metastasis en ABSTRACT Downregulation of tumor-suppressive microRNAs in breast cancer (BC) triggers molecular disturbances underlying cancer initiation, disease progression and metastasis. However, key microRNAs players acting as vital breaks on BC aggressiveness, with crucial regulatory roles in cell migration, epithelial-mesenchymal transition (EMT), invasion, and metas-tasis, remain underinvestigated. Our goal was to uncover candidate tumor-suppresive microRNAs associated with metastatic potential of BC via an in silico approach, which combined data from microRNA profiling studies on cell lines relevant to BC invasiveness, as well as on primary vs. metastatic BC. A minimal set of candidate microRNAs was determined from the intersection of downregulated sets from multiple eligible studies, confirmed by qPCR, assessed for potential clinical relevance in BC and functionally characterized. Predicted and experimentally validated targets of these microRNAs were identified (MiRTargetLink) and subjected to gene ontology (GO), KEGG pathway, and cancer hallmark enrichment analysis. microRNAs, invasion, EMT, metastasis, BRCA 1552100 978-86-82679-17-2 2026-08-27T08:16:48.031Z 44 yes 46 Aleksandra Nikezić Faculty of Science, University of Kragujevac Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor nauka-biološke nauke person 0000-0002-4652-7719 Sanja Goč University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor biohemijskih nauka person 0000-0002-6104-693X Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Miloš Brkušanin University of Belgrade-Faculty of Biology person Jovana Stevanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia person 0000-0002-3619-8472 Jovana Jovankić Faculty of Science, University of Kragujevac person application/pdf 3357748 https://phaidrabg.bg.ac.rs/o:39512 no yes 18 70 92000004 11A02 11A29 6th Belgrade Bioinformatics Conference-Book of Abstracts Institut za molekularnu genetiku i genetičko inženjerstvo, Univerzitet u Beogradu, Beograd, Srbija 2026