application/pdf 3357748 bytes 6th Belgrade Bioinformatics Conference-Book of Abstracts ABSTRACT Downregulation of tumor-suppressive microRNAs in breast cancer (BC) triggers molecular disturbances underlying cancer initiation, disease progression and metastasis. However, key microRNAs players acting as vital breaks on BC aggressiveness, with crucial regulatory roles in cell migration, epithelial-mesenchymal transition (EMT), invasion, and metas-tasis, remain underinvestigated. Our goal was to uncover candidate tumor-suppresive microRNAs associated with metastatic potential of BC via an in silico approach, which combined data from microRNA profiling studies on cell lines relevant to BC invasiveness, as well as on primary vs. metastatic BC. A minimal set of candidate microRNAs was determined from the intersection of downregulated sets from multiple eligible studies, confirmed by qPCR, assessed for potential clinical relevance in BC and functionally characterized. Predicted and experimentally validated targets of these microRNAs were identified (MiRTargetLink) and subjected to gene ontology (GO), KEGG pathway, and cancer hallmark enrichment analysis. info:eu-repo/semantics/conferenceProceedings 2026 Nikezić, Aleksandra Dobrijević, Zorana Goč, Sanja Nedić, Olgica Brkušanin, Miloš Stevanović, Jovana Jovankić, Jovana Identification and functional characterization of tumor-suppressive microRNAs with potential key roles in breast cancer progression and metastasis microRNAs, invasion, EMT, metastasis, BRCA Institut za molekularnu genetiku i genetičko inženjerstvo, Univerzitet u Beogradu, Beograd, Srbija eng https://phaidrabg.bg.ac.rs/o:39512 ISBN: 978-86-82679-17-2 http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode