o:39340 Taxifolin Inhibits Invasion and Endovascular Differentiation of Extravillous Trophoblast HTR-8/SVneo Cells en ABSTRACT Adequate placental development and function, prerequisites for the development of a healthy fetus, rely on controlled trophoblast invasion into the decidua and remodeling of the spiral arteries. These tightly regulated processes involve epithelial–mesenchymal transition (EMT) and endovascular differentiation of trophoblast cells. Taxifolin (dihydroquercetin), a natural flavonoid with various pharmacological effects, previously showed cytoprotective, antioxidant, and anti-inflammatory activity on trophoblast cells. Given that the literature indicates that this flavonoid suppresses EMT and can affect angiogenesis across different cell types, we investigated the potential of taxifolin (10 and 100 µM) to modulate invasion and endothelial-like differentiation in human extravillous trophoblast HTR-8/SVneo cells by functional tests. Expression of different molecular markers relevant to these processes was evaluated at the mRNA and protein levels. taxifolin; extravillous trophoblast; trophoblast invasion; endovascular differentiation; epithelial-mesenchymal transition 1552099 10.3390/cells15131152 2026-07-12T13:13:07.493Z 44 yes 46 Aleksandra Vilotić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka 0000-0002-0898-5401 Janko Legner University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia person 0000-0003-3720-1933 Žanka Bojić-Trbojević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka person 0000-0001-5619-1027 Marija Bruić Faculty of Pharmacy, Department of Pathobiology, University of Belgrade, Belgrade, Serbia person Biljana Spremo-Potparević Faculty of Pharmacy, Department of Pathobiology, University of Belgrade, Belgrade, Serbia person Milica Jovanović Krivokuća University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka person 0000-0001-8292-4853 Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka person 0000-0002-1182-4895 application/pdf 1974030 https://phaidrabg.bg.ac.rs/o:39340 no yes 16 70 92000001 11A29 11A13 Cells 15 13 MDPI, Basel, Switzerland 2026