Vilotić, Aleksandra Legner, Janko Bojić-Trbojević, Žanka Bruić, Marija Spremo-Potparević, Biljana Jovanović Krivokuća, Milica Pirković, Andrea eng info:eu-repo/semantics/article application/pdf 1974030 bytes https://phaidrabg.bg.ac.rs/o:39340 doi:10.3390/cells15131152 2026 http://creativecommons.org/licenses/by/4.0/legalcode taxifolin; extravillous trophoblast; trophoblast invasion; endovascular differentiation; epithelial-mesenchymal transition MDPI, Basel, Switzerland Taxifolin Inhibits Invasion and Endovascular Differentiation of Extravillous Trophoblast HTR-8/SVneo Cells ABSTRACT Adequate placental development and function, prerequisites for the development of a healthy fetus, rely on controlled trophoblast invasion into the decidua and remodeling of the spiral arteries. These tightly regulated processes involve epithelial–mesenchymal transition (EMT) and endovascular differentiation of trophoblast cells. Taxifolin (dihydroquercetin), a natural flavonoid with various pharmacological effects, previously showed cytoprotective, antioxidant, and anti-inflammatory activity on trophoblast cells. Given that the literature indicates that this flavonoid suppresses EMT and can affect angiogenesis across different cell types, we investigated the potential of taxifolin (10 and 100 µM) to modulate invasion and endothelial-like differentiation in human extravillous trophoblast HTR-8/SVneo cells by functional tests. Expression of different molecular markers relevant to these processes was evaluated at the mRNA and protein levels. Cells volume: 15 number: 13