o:38964 Strawberry Fragaria x ananassa Duchesne (cv. Romina) methanolic extract attenuates hydrogen peroxide induced DNA damage in human peripheral blood mononuclear cells in vitro en ABSTRACT Among berry fruits, strawberries are known for a wide array of beneficial biological activities, including antioxidant, antihypertensive, antibacterial, anti-proliferative, anti-inflammatory, anticancer, and anti-metastatic effects. The cultivar Fragaria × ananassa Duchesne (Romina) is particularly rich in bioactive constituents that may contribute to limiting DNA damage triggered by reactive oxygen species. In this study, we examined whether the methanolic extract of Romina strawberries (RE) exerted any genotoxic effect on human peripheral blood mononuclear cells (PBMCs) across concentrations ranging from 25 to 200 μg/mL using the comet assay. No evidence of genotoxicity was detected. Subsequently, the capacity of RE to counteract oxidative DNA damage in two experimental models was assesed. When PBMCs were pretreated with RE prior to hydrogen peroxide exposure, a slight reduction in DNA damage was observed, with only the 50 μg/mL concentration producing a statistically significant protective effect. ageing , comet assay, DNA damage, neurodegenerative disease 1552099 10.1177/1973798X261418527 2026-05-15T07:24:00.382Z 44 yes 46 Lada Živković Department of Pathobiology, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia Andrea Pirković Department for Biology of Reproduction, University of Belgrade, Institute for the Application of Nuclear Energy, Belgrade, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Dijana Topalović Department of Pathobiology, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia person Francesca Giampieri Department of Clinical Sciences, Polytechnic University of Marche, Ancona, Italy person Yasmany Armas Diaz Department of Clinical Sciences, Polytechnic University of Marche, Ancona, Italy person Mirta Milić Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia person Biljana Spremo-Potparević Department of Pathobiology, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia person application/pdf 129015 https://phaidrabg.bg.ac.rs/o:38964 no yes 17 70 92000001 11A13 11A29 11A2901 Mediterranean Journal of Nutrition and Metabolism 19 2 SAGE Publishing California, USA 2026