Mediterranean Journal of Nutrition and Metabolism volume: 19 number: 2 http://creativecommons.org/licenses/by-nc/4.0/legalcode SAGE Publishing California, USA info:eu-repo/semantics/article 2026 eng https://phaidrabg.bg.ac.rs/o:38964 doi:10.1177/1973798X261418527 ABSTRACT Among berry fruits, strawberries are known for a wide array of beneficial biological activities, including antioxidant, antihypertensive, antibacterial, anti-proliferative, anti-inflammatory, anticancer, and anti-metastatic effects. The cultivar Fragaria × ananassa Duchesne (Romina) is particularly rich in bioactive constituents that may contribute to limiting DNA damage triggered by reactive oxygen species. In this study, we examined whether the methanolic extract of Romina strawberries (RE) exerted any genotoxic effect on human peripheral blood mononuclear cells (PBMCs) across concentrations ranging from 25 to 200 μg/mL using the comet assay. No evidence of genotoxicity was detected. Subsequently, the capacity of RE to counteract oxidative DNA damage in two experimental models was assesed. When PBMCs were pretreated with RE prior to hydrogen peroxide exposure, a slight reduction in DNA damage was observed, with only the 50 μg/mL concentration producing a statistically significant protective effect. ageing , comet assay, DNA damage, neurodegenerative disease application/pdf 129015 bytes Živković, Lada Pirković, Andrea Topalović, Dijana Giampieri, Francesca Armas Diaz, Yasmany Milić, Mirta Spremo-Potparević, Biljana Strawberry Fragaria x ananassa Duchesne (cv. Romina) methanolic extract attenuates hydrogen peroxide induced DNA damage in human peripheral blood mononuclear cells in vitro