o:37115 Caveolin-1 as potential biomarkers of prostate cancer Evidence from Serbian population en Caveolin-1, a membrane scaffolding protein, plays a key role in regulating cell proliferation, migration, and angiogenesis. Its overexpression in prostate cancer (PCa) cells is significantly associated with increased tumor aggressiveness. The aim of this study was to investigate whether caveolin-1, found in plasma-derived exosomes, could serve as a liquid biopsy biomarker for PCa. Exosomes were isolated from plasma samples of 39 patients with PCa and 33 patients with benign prostatic hyperplasia (BPH), used as the control group, using the Total Exosome Isolation Kit (from plasma) according to the manufacturer’s instructions. Morphological characterization of the isolated exosomes was performed using transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking analysis (NTA), and flow cytometry. Western blot analysis confirmed the presence of the typical exosomal marker CD63, as well as caveolin-1 in plasma exosomes. The results showed no statistically significant difference in exosomal caveolin-1 levels between PCa and BPH patients (p=0.349). However, when PCa patients were stratified according to standard prognostic parameters and tumor aggressiveness scores, the relative protein levels differed between subgroups. exosomes, liquid biopsy, prostatic hyperplasia 1552100 978-86-7220-136-9 2025-11-21T13:03:18.986Z 44 yes 46 Suzana Matijašević Joković Center for Human Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, Serbia Aleksandra Korać Centre for Electron Microscopy, Faculty of Biology, University of Belgrade, Belgrade, Serbia person Sanja Kovačević Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, Department of Biochemistry, University of Belgrade, Belgrade, Serbia person Ana Đorđević Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, Department of Biochemistry, University of Belgrade, Belgrade, Serbia person Lidija Filipović Innovative Centre, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia person Zorana Dobrijević Institute for the Application of Nuclear Energy INEP, University of Belgrade, Serbia doktor biohemijskih nauka person 0000-0002-4652-7719 Duška Savić-Pavićević Center for Human Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, Serbia person Ivan Vuković Clinic of Urology, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia person Milica Popović Faculty of Chemistry, University of Belgrade, Belgrade, Serbia person Goran Brajušković Center for Human Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, Serbia person application/pdf 615831 https://phaidrabg.bg.ac.rs/o:37115 no yes 18 70 92000004 11A02 11A21 11A19 11A29 Caveolin-1 as potential biomarkers of prostate cancer: Evidence from Serbian population 131 132 FEBS3+ Meeting "Advances in Molecular Biosciences: From Genes to Personalized Therapies" – Book of Abstracts Beograd, Srbija Hemijski fakultet, Srpsko biohemijsko društvo 2025