Caveolin-1 as potential biomarkers of prostate cancer: Evidence from Serbian population startpage: 131 endpage: 132 exosomes, liquid biopsy, prostatic hyperplasia 2025 info:eu-repo/semantics/conferenceProceedings Caveolin-1, a membrane scaffolding protein, plays a key role in regulating cell proliferation, migration, and angiogenesis. Its overexpression in prostate cancer (PCa) cells is significantly associated with increased tumor aggressiveness. The aim of this study was to investigate whether caveolin-1, found in plasma-derived exosomes, could serve as a liquid biopsy biomarker for PCa. Exosomes were isolated from plasma samples of 39 patients with PCa and 33 patients with benign prostatic hyperplasia (BPH), used as the control group, using the Total Exosome Isolation Kit (from plasma) according to the manufacturer’s instructions. Morphological characterization of the isolated exosomes was performed using transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking analysis (NTA), and flow cytometry. Western blot analysis confirmed the presence of the typical exosomal marker CD63, as well as caveolin-1 in plasma exosomes. The results showed no statistically significant difference in exosomal caveolin-1 levels between PCa and BPH patients (p=0.349). However, when PCa patients were stratified according to standard prognostic parameters and tumor aggressiveness scores, the relative protein levels differed between subgroups. Caveolin-1 as potential biomarkers of prostate cancer Evidence from Serbian population Matijašević Joković, Suzana Korać, Aleksandra Kovačević, Sanja Đorđević, Ana Filipović, Lidija Dobrijević, Zorana Savić-Pavićević, Duška Vuković, Ivan Popović, Milica Brajušković, Goran http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode eng Hemijski fakultet, Srpsko biohemijsko društvo application/pdf 615831 bytes https://phaidrabg.bg.ac.rs/o:37115 ISBN: 978-86-7220-136-9