o:36893 Profilaktičko dejstvo rekombinantne izoforme lektina banane u zapaljenskim bolestima creva: ispitivanje na modelu hemijski-indukovanog kolitisa u miša doktorska disertacija Prophylactic effect of recombinant banana lectin in inflammatory bowel diseases: evaluation in a murine model of chemically induced colitis : doctoral dissertation sr Inflamatorne bolesti creva (IBD), uključujući ulcerozni kolitis i Kronovu bolest, sve suučestalije širom sveta i značajno narušavaju kvalitet života obolelih. Patogeneza ovih bolesti rezultatje složene interakcije genetskih faktora, crevne mikrobiote i uticaja iz spoljašnje sredine. Cilj ovedoktorske teze bio je da se ispita efekat rekombinantne izoforme lektina banane (rBanLec), poznatogpo svojim imunomodulatornim svojstvima, u eksperimentalnom modelu 2,4,6-trinitrobenzensulfonskom kiselinom-izazvanog kolitisa u C57BL/6 mišu. rBanLec je primenjivan u dozama od 0,1,1 i 10 μg/mL (100 μL) pre ili nakon izazivanja eksperimentalnog kolitisa. Težina bolesti procenjivanaje na osnovu gubitka telesne težine, skraćenja dužine debelog creva, histopatoloških promena,citokinskog profila i parametara oksidativnog stresa u debelom crevu, kao i lokalnih promena upopulaciji imunskih ćelija. Najpovoljniji efekat je postignut primenom 0,1 μg/mL rBanLec, kakojednokratno profilaktički tako i tokom bolesti. Pozitivan efekat primene rBanLec se ogledao uznačajnom ublaženju kliničke slike u piku bolesti i ubrzavanju oporavka, a bio je povezan sasmanjenjem intenziteta histoloških promena, povećanjem lokalne koncentracije antiinflamatornihcitokina i pojačanom aktivnošću antioksidativnih enzima u debelom crevu. Rezultati ukazuju daprimena rBanLec u niskim doza može imati povoljno dejstvo u IBD putem modulacije oksidativnogstresa i imunskog odgovora, što dalje ukazuje na potencijalnu mogućnost primene u kontroli IBD. The incidence of inflammatory bowel diseases (IBD), such as ulcerative colitis and Crohn’sdisease, continues to increase globally. Given the profound impact of IBDs on patient quality of life,the development of effective preventative and therapeutic strategies remains a critical priority. IBDdevelops through the combined influence of genetics, gut microbial composition, and environmentaltriggers. The study utilized a TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced colitis model inC57BL/6 mice to examine the effects of recombinant banan lectin (rBanLec), a mannose-specificlectin known for its immunomodulatory activity. Mice received rBanLec at concentrations of 0.1, 1,and 10 μg/mL (100 μL) prior or after to colitis induction. Disease severity was evaluated throughparameters such as body weight loss, colon shortening, histopathological analysis, cytokine profiling,oxidative stress biomarker assessment and local changes in immune cell populations. Treatment withthe lowest tested dose of rBanLec (0.1 μg/mL) led to a significant reduction in colitis severity, asevidenced by less weight loss, improved histological outcomes, and a shift toward an antiinflammatorycytokine profile. Furthermore, rBanLec enhanced the activity of key antioxidantenzymes and lowered oxidative stress indicators during peak inflammation. These results suggest thatlow-dose rBanLec has a protective role in experimental colitis through its ability to attenuateoxidative damage and promote anti-inflammatory immune responses. This positions rBanLec as apromising candidate for future interventions in control of IBD pathology. Biohemija - Imunologija / Biochemistry - Immunology Datum odbrane: 14.10.2025. IBD, lektin banane (BanLec), imunomodulacija, antiinflamatorna aktivnost, debelo crevo, TNBS izazvani kolitis, citokini, antioksidativni enzimi, C57BL/6 miševi IBD, banana lectin (BanLec), immunomodulation, anti-inflammatory, colon, TNBSinduced colitis, citokines, antioxidative enzymes, C57BL/6 mice 91552100 178612745 91552101 9942 2025-10-30T10:11:48.053Z 45 no 46 Radmila D., 1990- Miljković 130276105 2025 63 mentor Marijana, 1973- Stojanović 9691239 2025 63 mentor Marija, 1969- Gavrović-Jankulović 12778343 2025 63 član komisije Milica, 1982- Popović 13876583 2025 63 član komisije Marija, 1986- Stojadinović 14049895 2025 63 član komisije Ivana, 1984- Prodić 17949287 2025 100 str. 6350702 http://phaidrabg.bg.ac.rs/o:36893 no yes 13 2025-10-30T10:11:48.320Z 70 11 1066841 1066866 IBD, lektin banane (BanLec), imunomodulacija, antiinflamatorna aktivnost, debelocrevo, TNBS izazvani kolitis, citokini, antioksidativni enzimi, C57BL/6 miševi IBD, banana lectin (BanLec), immunomodulation, anti-inflammatory, colon, TNBSinducedcolitis, citokines, antioxidative enzymes, C57BL/6 mice 577.27:616.34-002(043.3) 1738 11A19 2025