o:36610 Identification of potential microRNA biomarkers for early diagnosis of hepatocellular carcinoma applying bioinformatics approaches en Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide, largely due to limited tools for early detection and the asymptomatic nature of early stage disease. Circulating microRNAs (miRNAs) have emerged as promising non‐invasive biomarkers because of their high stability in body fluids and disease‐specific expression patterns. In this study, we performed an in silico analysis of serum miRNA expression profiles using the Gene Expression Omnibus (GEO) dataset GSE113740, which included 40 patients with HCC and 10 healthy controls. A total of 257 differentially expressed miRNAs were identified, of which seven (hsa‐miR‐885‐3p, hsa‐miR‐320a, hsa‐miR‐744‐5p, hsa‐miR‐221‐3p, hsa‐miR‐561‐3p, hsa‐miR‐124‐3p, and hsa‐miR‐637) were prioritized based on network metrics, including the number of single‐line regulators (NSR) and transcription factor percentage (TFR) (p < 0.05). The majority have previously been reported as functionally associated with HCC. Meta‐profile analysis across 40 cancer types revealed distinct liver cancer‐specific expression for five of these miRNAs (hsa-miR-885-3p, hsa-miR-320a, hsa-miR-744-5p, has-miR-221-3p, and has-miR-124-3p). Network topology and pathway enrichment highlighted key regulatory hubs and cancer‐related targets. Ultimately, four miRNAs (hsa‐miR‐885‐3p, hsa‐miR‐320a, hsa‐miR‐744‐5p, and hsa‐miR‐124‐3p) emerged as promising diagnostic biomarkers for early HCC detection. hepatocellular carcinoma, microRNA, biomarker 1552099 10.46793/ICCBIKG25.288G 2025-10-02T11:07:14.848Z 44 yes 46 Sanja Goč University of Belgrade, Institute for the Application of Nuclear Energy, Serbia dr bioloških nauka 0000-0002-6104-693X Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia dr bioloških nauka person 0000-0002-4652-7719 Tamara Janković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia Magistar farmacije-medicinski biohemičar person 0000-0002-8188-0921 Filip Janjić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia dr medicinskih nauka-veterinarska medicina person 0000-0002-5121-1145 Ninoslav Mitić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia dr bioloških nauka person 0000-0003-2756-6517 Miroslava Janković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia dr bioloških nauka person 0000-0002-7889-5110 application/pdf 10208205 https://phaidrabg.bg.ac.rs/o:36610 no yes 18 70 92000004 11A29 Identification of potential microRNA biomarkers for early diagnosis of hepatocellular carcinoma applying bioinformatics approaches 289 293 3rd International Conference on Chemo and BioInformatics, Book of Proceedings Institut za informacione tehnologije, Univerzitet u Kragujevcu, Srbija 2025