application/pdf 5362814 bytes info:eu-repo/semantics/article Šavikin, Katarina Alimpić Aradski, Ana Jovanović, Aleksandra A. Pirković, Andrea Radan, Milica Ćujić-Nikolić, Nada Živković, Jelena Elsevier 2025 dan http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode From food waste to anti-aging solution: Harnessing the bioactive potential of pomegranate peel microparticles in skincare volume: 164 number: 108818 Punica granatum Phenolics UV radiation Skin-related enzymes Ellagitannins Molecular docking Anti-inflammatory https://phaidrabg.bg.ac.rs/o:36523 doi:https://doi.org/10.1016/j.bioorg.2025.108818 Pomegranate peel (PP), comprising over 50 % of the fruit’s weight, is often discarded as waste despite its rich composition, particularly in ellagitannins. This study explores the potential of microencapsulated PP (MPP) extract in managing skin conditions related to inflammation and enzymatic aging processes. Additionally, the impact of UV irradiation on its chemical profile was assessed to determine its suitability for skincare applications. HPLC and FT-IR analyses confirmed that UVA, UVB, and UVC irradiation did not alter the chemical composition of MPP. The major identified ellagitannins were punicalagin (118.01 mg/g dry weight) and punicalin (37.38 mg/g dry weight), alongside significant amounts of gallic and ellagic acids. In vitro studies demonstrated that MPP exhibited inhibitory activity against collagenase, elastase, hyaluronidase, and tyrosinase, suggesting promising anti-aging potential. These findings were further supported by a molecular docking study, which revealed key interactions between MPP bioactives and enzyme active sites. MPP showed dose-dependent UVA and UVB absorption comparable to zinc oxide. In human keratinocytes (HaCaT), MPP reduced reactive oxygen species production in the presence of H₂O₂, and IL-1β and MIF expressions, confirming the anti-inflammatory effect against lipopolysaccharide challenge. Additionally, MPP in all tested concentrations (50–200 μg/mL), protected erythrocyte membranes from heat- and hypotonic-induced lysis, demonstrating concentration-dependent anti-inflammatory (membrane stability) effects. Lower MPP concentrations did not significantly affect keratinocyte viability. These findings highlight MPP’s potential for use in cosmetic and pharmaceutical skincare formulations targeting inflammation, oxidative stress, and aging-related concerns. From food waste to anti-aging solution: Harnessing the bioactive potential of pomegranate peel microparticles in skincare