o:36449 Upregulation of the Antioxidant Response-Related microRNAs miR-146a-5p and miR-21-5p in Gestational Diabetes: An Analysis of Matched Samples of Extracellular Vesicles and PBMCs Povećana ekspresija mikro RNK miR-146a-5p i miR-21-5p uključenih u regulaciju antioksidativnog odgovora u gestacijskom dijabetesu: analiza sparenih uzoraka ekstraćelijskih vezikula i mononuklearnih ćelija periferne krvi en ABSTRACT MicroRNA-based regulatory mechanisms show disturbances related to oxidative stress (OS) interconnected with inflammation (IFM), as well as impairments associated with gestational diabetes (GDM). The aim of this study was to assess the diagnostic and prognostic significance of the OS/IFM-related microRNA in GDM by using peripheral blood mononuclear cells (PBMCs) and serum-derived extracellular vesicles (EVs) as biological samples. We selected the known OS/IFM-associated microRNAs miR-146a-5p, miR-155-5p, and miR-21-5p as candidates for our GDM biomarker analysis. miR-21-5p; miR-155-5p; miR-146a-5p; oxidative stress; redox status; gestational diabetes; iron; superoxide dismutase; adverse outcomes 1552099 10.3390/ijms26146902 2025-08-06T07:23:55.105Z 44 yes 46 Jovana Stevanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia 0000-0002-3619-8472 Ninoslav Mitić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0003-2756-6517 Ana Penezić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2539-4507 Ognjen Radojičić Clinic for Gynecology and Obstetrics "Narodni Front", Belgrade, Serbia person Daniela Ardalić Clinic for Gynecology and Obstetrics "Narodni Front", Belgrade, Serbia person Milica Mandić Clinic for Gynecology and Obstetrics "Narodni Front", Belgrade, Serbia person Vesna Mandić-Marković Medical School, University of Belgrade, Belgrade, Serbia person Željko Miković Medical School, University of Belgrade, Belgrade, Serbia person Miloš Brkušanin Centre for Human Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, Serbia person Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 application/pdf 3429421 https://phaidrabg.bg.ac.rs/o:36449 no yes 16 70 92000001 11A29 11A2905 11A32 11A02 International Journal of Molecular Sciences 26 14 6902 MDPI, Basel, Switzerland 2025