https://phaidrabg.bg.ac.rs/o:36383 doi:10.3390/cimb47060420 application/pdf 368785 bytes ABSTRACT Chromosomal instability in Alzheimer’s disease (AD) neurons has been previously reported. This pilot study aimed to establish a quantitative technique for assessing X chromosome centromere signals using fluorescence in situ hybridization (FISH). Hippocampal brain tissue was collected at autopsy from sporadic AD patients and age- and gender-matched controls. FISH was utilized to detect and measure the intensity of hybridization signals for X chromosome centromeres in the interphase nuclei of hippocampal brain cells. The premature centromere division (PCD) phenomenon, marked by a close bipartite signal appearing as two separated FISH spots, was examined to see if the hybridized DNA amount in each spot matched the expected centromere DNA amount. Spremo-Potparević, Biljana Popović, Petar Topalović, Dijana Pirković, Andrea Perry, George Živković, Lada info:eu-repo/semantics/article http://creativecommons.org/licenses/by/4.0/legalcode Alzheimer’s disease; premature centromere division; X chromosome; chromosomal instability; fluorescence in situ hybridization Assessment of X Chromosome Centromere Instability in Alzheimer’s Disease: A Quantitative FISH Approach Current Issues Molecular Biology volume: 47 number: 6 2025 MDPI, Basel, Switzerland eng