o:36382 Human Monocytes Exposed to SARS-CoV-2 Display Features of Innate Immune Memory Producing High Levels of CXCL10 upon Restimulation Humani monociti izloženi SARS-CoV-2 pokazuju karakteristike memorije urodjenog imunskog sistema i proizvode visoke nivoe CXCL10 nakon restimulacije en ABSTRACT Introduction: A role for innate immune memory in protection during COVID-19 infection or vaccination has been recently reported. However, no study so far has shown whether the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can train innate immune cells. The aim of this study was to investigate whether this virus can induce trained immunity in human monocytes. Methods: Monocytes were exposed to inactivated SARS-CoV-2 (iSARS-CoV-2) for 24 h, followed by a resting period in the medium only and a secondary stimulation on day 6 after which the cytokine/chemokine and transcriptomic profiles were determined. Trained immunity, Severe acute respiratory syndrome coronavirus 2, CXCL10, Monocytes 1552099 10.1159/000535120 2025-07-07T10:31:49.589Z 44 yes 46 Jelena Cvetković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka 0000-0003-0481-3669 Ronald H.J. Jacobi Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands person Alberto Miranda-Bedate Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands person Nhung Pham Department of Bioinformatics – BiGCaT, NUTRIM, Maastricht University, Maastricht, The Netherlands person Martina Kutmon Maastricht Centre for Systems Biology (MaCSBio), Maastricht University, Maastricht, The Netherlands person James Groot Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands person Martijn D.B. van de Garde Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands person Elena Pinelli Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands person application/pdf 1151977 https://phaidrabg.bg.ac.rs/o:36382 no yes 17 70 92000001 11A29 Journal of Innate Immunity 15 1 911 924 S. Karger AG, Basel 2023