o:36375 Implication of p16 Promoter Methylation, the BRAFV600E Mutation, and ETS1 Expression Determination on Papillary Thyroid Carcinoma Prognosis and High-Risk Patients’ Selection Implikacije odredjivanja metilacije promotora p16, mutacije BRAFV600E i ekspresije ETS1 na prognozu papilarnog karcinoma štitaste žlezde i identifikaciju visoko rizičnih pacijenata en ABSTRACT Background/Objectives: Papillary thyroid carcinoma (PTC) is the most common malignancy of the endocrine system, characterized by various molecular alterations. This study evaluates the relationship between p16 promoter methylation status, BRAFV600E mutation presence, and ETS1 (E26 transformation-specific) expression, aiming to better understand their clinical significance and to enhance the risk stratification of PTC patients. Methods: p16 promoter methylation was analyzed by methylation-specific PCR (MSP), BRAFV600E by mutant allele-specific PCR amplification (MASA), ETS1 mRNA expression by quantitative PCR (qPCR), ETS1 protein expression by immunohistochemistry (IHC), and Western blot. All tested factors were further associated with the occurrence of unfavorable clinicopathological data of the patients. tumor marker; prediction; tumor aggressiveness; epigenetic; PTC; patients’ stratification 1552099 10.3390/biomedicines13071583 2025-07-02T12:53:40.839Z 44 yes 46 Stefana Stojanović Novković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia 0000-0003-3808-0467 Sonja Šelemetjev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-2898-6671 Milena Krajnović University of Belgrade, VINČA Institute of Nuclear Sciences, National Institute of the Republic of Serbia, Belgrade, Serbia person Ana Božović University of Belgrade, VINČA Institute of Nuclear Sciences, National Institute of the Republic of Serbia, Belgrade, Serbia person Bojana Kožik University of Belgrade, VINČA Institute of Nuclear Sciences, National Institute of the Republic of Serbia, Belgrade, Serbia person Uršula Prosenc Zmrzljak Molecular Biology Laboratory, BIA Separations CRO-Labena d.o.o, Ljubljana, Slovenia person Tijana Išić Denčić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8475-2266 application/pdf 954761 https://phaidrabg.bg.ac.rs/o:36375 no yes 16 70 92000001 11A29 11A2902 11A28 11A2810 Biomedicines 13 7 1583 MDPI, Basel, Switzerland 2025