o:36343 Radical scavenging activity of silymarin encapsulated in liposomal vesicles: impact of UV irradiation and lyophilization en The radical scavenging activity of silymarin-loaded liposomes after different technological processes (UV irradiation and lyophilization) was investigated using DPPH and ABTS assays. Using the DPPH method, the antioxidant capacity of pure silymarin was 84.03%, while it was lower after encapsulation in liposomes; it was 81.63% after the formulation, 81.15% after UV irradiation, and 79.85% after lyophilization. The anti-ABTS potential was 3.04 µmol Trolox equivalent (TE)/mL for silymarin, 1.68 µmol TE/mL after the liposome preparation, 1.52 µmol TE/mL after UV irradiation, and 2.02 µmol TE/mL after lyophilization. UV irradiation did not cause significant changes in the antioxidant potential of liposomes, while the ABTS scavenging activity was higher after lyophilization. Considering that the two used antioxidant assays are based on different reactions, the obtained data provide a good insight into the overall antioxidant activity of silymarin-loaded liposomes. antioxidants; liposomes; silymarin; lyophilization; UV irradiation 1552099 https://doi.org/10.3390/engproc2025099014 2025-06-18T17:07:31.864Z 44 yes 46 Amjed Karkad Faculty of Technology and Metallurgy University of Belgrade Milena Milosevic Institute of Chemistry, Technology and Metallurgy—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia person Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy doktor bioloških nauka person 0000-0002-1182-4895 Aleksandar Marinkovic Faculty of Technology and Metallurgy, University of Belgrade person Aleksandra Jovanović Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 196302 https://phaidrabg.bg.ac.rs/o:36343 no yes 16 70 92000004 11A29 11A41 11A24 Radical scavenging activity of silymarin encapsulated in liposomal vesicles: impact of UV irradiation and lyophilization 99 1 14 Engineering Proceedings Basel, Switzerland MDPI 2025