o:36338 Satureja montana extracts: the impact of temperature in microwave-assisted extraction en Aromatic plants of the Lamiaceae family are valuable food and medicinal sources due to their antioxidant, antimicrobial, antispasmodic, expectorant, carminative, antiulcer, stimulant, anticancer, anti-proliferative, and diuretic effects. Biologically active compounds obtained from plant resources have gained more attention nowadays, due to the consumerism demand for functional food and pharmaceuticals based on natural compounds. In the present study, Satureja montana herba was evaluated as a source of antioxidant polyphenol components, which were extracted by the application of a microwave assisted extraction device. Microwave-assisted extraction has become a good alternative extraction procedure in comparison to traditional techniques due to its high efficiency, faster kinetics, and reduced extraction medium consumption, which is in accordance with the aim of the manufacturing sector to seek out eco-innovative technologies, which minimize the loss of bioactive compounds. Optimization of the extraction was carried out by varying the extraction temperature (60-200°C) in 50% ethanol, at a 1:40 solid-to-solvent ratio for 150 s. The extraction efficiency was expressed in terms of total polyphenol content (TPC), total flavonoid content (TFC), and DPPH radical scavenging capacity (expressed as the IC50, the concentration of the extract requires to neutralize 50% of free radicals). The highest TPC was recorded in the extract prepared at 160°C (1.77±0.14 mg gallic acid equivalent - GAE/g dry extract), while the highest TFC was in the extract obtained at 180°C (0.39±0.01 mg catechin equivalent - CE/g dry extract). Also, the level of polyphenol yield was correlated with free radical scavenging activity and the IC50 value was the lowest (the highest antioxidant activity, 5.30±0.21 mg/mL) in the sample with the highest TPC. This study revealed that polyphenol and flavonoid yields and antioxidant potential of S. montana extracts were significantly affected by the temperature in the microwave-assisted extraction. This is the first step in obtaining the highest amount of bioactive compounds from S. montana that can be used in the food, pharmaceutical, or cosmetic industry, in their free form or encapsulated into adequate carriers. winter savory, bioactive compounds, extracts 1552100 978-99955-81-52-7 2025-06-18T12:27:25.844Z 44 yes 46 Natalija Čutović Institute for Medicinal Plant Research “Dr. Josif Pančić”, Serbia, Belgrade Tatjana Marković Institute for Medicinal Plants Research “Dr. Josif Pančić“, Belgrade, Serbia person Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy doktor tehnoloških nauka person 0000-0001-5394-0125 Petar Batinić Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Koscuska 1, Serbia, Belgrade person application/pdf 4285659 https://phaidrabg.bg.ac.rs/o:36338 no yes 16 70 92000005 11A29 Satureja montana extracts: the impact of temperature in microwave-assisted extraction 9 1 2 Book of Abstracts of EEM2025 Karakaj 34a, 75 400 Zvornik Republic of Srpska, B&H UNIVERSITY OF EAST SARAJEVO FACULTY OF TECHNOLOGY 2025