o:36335 Application of Lion's Mane Mushroom in Cosmetics: The Influence of Drying Method and Solvent en Introduction: Mushrooms have traditionally been studied for their nutritional and medicinal properties; however, growing interest has recently emerged in their potential applications in cosmetics. Increasing evidence suggests that mushrooms are a rich source of valuable cosmeceuticals, particularly compounds with anti-aging properties, which are among the most sought-after in skincare. As the market for mushroom-based cosmetics continues to expand, there is a clear need for further scientific research in this area. Lion’s mane mushroom (Hericium erinaceus) is a fungi species that parasites on oaks and produces characteristic irpicoid fruiting bodies; in recent years, it has gained popularity as a commercially cultivated edible species with potential health benefits. The aim of this study was to investigate the potential cosmetic benefits of lion’s mane mushroom. Experimental: The effect of drying was assessed using two methods: heat drying (38°C, 24 h) and freeze-drying (-40°C, 0.12 mbar, 24 h). Additionally, the influence of solvent polarity was evaluated using water and water-ethanol mixtures containing 30%, 50%, and 70% ethanol. The resulting extracts were subjected to chemical and biological characterization, with a focus on compounds and activities beneficial for skincare. Total carbohydrates were quantified via the phenol-sulfuric acid method. Sugars and polyols were analyzed using HPLC, and glucan content was determined with a Megazyme® β-Glucan Assay Kit. Antioxidant activity was assessed in vitro using ABTS, CuPRAC, and β-carotene bleaching assays. Antityrosinase activity was evaluated via an in vitro enzymatic assay. 1552100 978-86-7401-396-0 2025-06-18T11:30:23.681Z 44 yes 46 Anja Malović Faculty of Technology and Metallurgy, University of Belgrade Joka Nikolić Faculty of Technology and Metallurgy, University of Belgrade person Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy doktor tehnoloških nauka person 0000-0001-5394-0125 Rada Pjanovic Faculty of Technology and Metallurgy, University of Belgrade person Katarina Banjanac Innovation Centre of the Faculty of Technology and Metallurgy, University of Belgrade person Milica Veljković Innovation Centre of the Faculty of Technology and Metallurgy, University of Belgrade person Verica Djordjevic Faculty of Technology and Metallurgy, University of Belgrade person Predrag Petrovic Innovation Centre of the Faculty of Technology and Metallurgy, University of Belgrade person application/pdf 573120 https://phaidrabg.bg.ac.rs/o:36335 no yes 18 70 92000005 11A41 11A29 11A2910 Application of Lion's Mane Mushroom in Cosmetics: The Influence of Drying Method and Solvent 172-173 172 173 Book of Abstracts of BIOBASED FUTURE: GREEN BIOPROCESSING FOR INNOVATIVE BIOACTIVE PRODUCTS Karnegijeva 4, 11000 Belgrade, Serbia University of Belgrade, Faculty of Technology and Metallurgy 2025