o:36279 EVs from Trichinella spiralis muscle larvae exert immunomodulatory potential in chronic inflammatory Th2 disorders en Excretory-secretory products of Trichinella spiralis muscle larvae (ES L1) have immunomodulatory properties i.e. reduce hypersensitivity to both allergens and autoantigens. Recently, we have shown that extracellular vesicles isolated from ES L1 (TsEVs) exert immunomodulatory properties on human monocyte derived dendritic cells. Now, we aim to investigate whether TsEVs can also exert beneficial immunomodulatory effects in murine model of ovalbumin (OVA)-induced allergy. Methods: TsEVs were enriched from ES L1 by differential centrifugation and ultrafiltration. Experimental allergic airway inflammation was induced in BALB/c mice by intraperitoneal injection of OVA in alum on days 1 and 14. On days 21-24 mice were challenged with intranasal application of OVA, 30 min after intranasal administration of TsEVs or PBS and sacrificed two days later. Blood samples were taken for serum IgE determination. Lungs and spleens were extracted for the isolation of immune cells. Phenotype of immune cells was determined by flow cytometry and their cytokine production by ELISA assays. Results: TsEVs treatment of allergic mice lead to diminished numbers of alveolar macrophage and CD103+ dendritic cells (DC) in lungs compared to allergic control while numbers of CD11b+ DCs and their Ly6C+ subset was increased, along with CD8+ and CD19+ T cells. Upon restimulation with OVA, splenocytes and lung immune cells of TsEVs-treated mice produced lower levels of Th2 cytokines, while the production of IFN-g was elevated only in lung immune cells. Lower IgE levels were found in TsEVs-treated mice compared to sham-treated controls. Summary/Conclusion: TsEVs exert immunomodulatory properties inmurinemodel of allergic airway inflammation by diminishing inflammation and thus theymay represent the basis for novel allergen-independent therapeutics in treatment of respiratory allergies. 1552099 10.1002/jev2.12224 2025-06-10T08:46:22.159Z 44 yes 46 Sofija Glamočlija University of Belgrade, Institute for the Application of Nuclear Energy, Serbia master biolog 0000-0003-0497-5852 Anna Schmid Medical University of Vienna, Vienna, Austria person Nataša Ilić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-6738-7033 Alisa Gruden-Movsesijan University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-3061-5474 Saša Vasilev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor veterinarskih nauka person 0000-0001-7870-2713 Irma Schabussova Medical University of Vienna, Vienna, Austria person Maja Kosanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1143-1805 application/pdf 121264 https://phaidrabg.bg.ac.rs/o:36279 no yes 16 70 92000004 11A29 EVs fromTrichinella spiralis muscle larvae exert immunomodulatory potential in chronic inflammatory Th2 disorders PT05.07 149 150 ISEV2022 Abstract Book of Journal of Extracellular Vesicles Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles 2022