o:36244 Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility en ABSTRACT Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. electron microscopy, EV guidelines, EV reporting, extracellular vesicles, helminths, parasites 1552099 10.1002/jev2.12298 1552102 https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12298 2025-06-06T10:55:43.729Z 44 yes 46 Ruby White Javier Sotillo person María Eugenia Ancarola person Anne Borup person Anders Toftegaard Boysen person Paul J. Brindley person Edit I. Buzás person Serena Cavallero person et al. person Maja Kosanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1143-1805 application/pdf 5097154 https://phaidrabg.bg.ac.rs/o:36244 no yes 16 70 92000006 11A29 11A2904 Journal of Extracellular Vesicles 12 1 Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles 2023