o:36240 Minimal information for studies of extracellular vesicles (MISEV2023); From basic to advanced approaches en ABSTRACT Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. ctosomes, exosomes, extracellular vesicles, extracellular particles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation 1552099 10.1002/jev2.12404 1552102 https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12404 2025-06-06T09:02:52.866Z 44 yes 46 Joshua A. Welsh Deborah C. I. Goberdhan person Lorraine O'Driscoll person Edit I. Buzas person Cherie Blenkiron person Benedetta Bussolati person Houjian Cai person Dolores Di Vizio person Tom A. P. Driedonks person Uta Erdbrügger person Juan M. Falcon-Perez person Qing-Ling Fu person Andrew F. Hill person Metka Lenassi person Sai Kiang Lim person Mỹ G. Mahoney person Sujata Mohanty person Andreas Möller person Rienk Nieuwland person et al. person 63 Authorship and Participation Maja Kosanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka 0000-0002-1143-1805 Sofija Glamočlija University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0003-0497-5852 application/pdf 2581959 https://phaidrabg.bg.ac.rs/o:36240 no yes 16 70 92000006 11A29 11A2904 Journal of Extracellular Vesicles 13 2 Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles 2024