Welsh, Joshua A. Goberdhan, Deborah C. I. O'Driscoll, Lorraine Buzas, Edit I. Blenkiron, Cherie Bussolati, Benedetta Cai, Houjian Di Vizio, Dolores Driedonks, Tom A. P. Erdbrügger, Uta Falcon-Perez, Juan M. Fu, Qing-Ling Hill, Andrew F. Lenassi, Metka Kiang Lim, Sai Mahoney, Mỹ G. Mohanty, Sujata Möller, Andreas Nieuwland, Rienk , et al. Minimal information for studies of extracellular vesicles (MISEV2023); From basic to advanced approaches info:eu-repo/semantics/article http://creativecommons.org/licenses/by/4.0/legalcode eng Kosanović, Maja Glamočlija, Sofija Journal of Extracellular Vesicles volume: 13 number: 2 Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles ABSTRACT Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. 2024 application/pdf 2581959 bytes https://phaidrabg.bg.ac.rs/o:36240 doi:10.1002/jev2.12404 ctosomes, exosomes, extracellular vesicles, extracellular particles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation