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    <ns1:title language="en">Immunomodulatory Potential of Trophoblast-Released Extracellular Vesicles on Activated PBMCs</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
During pregnancy, trophoblasts express and secrete a battery of immunomodulatory molecules to sustain immune tolerance at the feto-maternal interface. The study aimed to investigate the effects of extracellular vesicles (EVs) released by first trimester extravillous trophoblast HTR-8/SVneo cell line on activated peripheral blood mononuclear cells (PBMCs), and their ability to modulate cellular proliferation and the expression of inflammatory molecules. EVs were isolated from HTR-8/SVneo cell-conditioned medium and characterized for size, quantity, and exosomal markers using nanoparticle tracking analysis (NTA) and Western blot, respectively. Subsequently, PBMCs were isolated from healthy human donors and activated by PHA. </ns1:description>
    <ns1:keyword language="en">extracellular vesicles, activated peripheral blood mononuclear cells (PBMCs)</ns1:keyword>
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    <ns1:upload_date>2025-06-05T07:47:45.726Z</ns1:upload_date>
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        <ns3:firstname>Andrea</ns3:firstname>
        <ns3:lastname>Pirković</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
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        <ns3:firstname>Aleksandra</ns3:firstname>
        <ns3:lastname>Vilotić</ns3:lastname>
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        <ns3:firstname>Milica</ns3:firstname>
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        <ns3:firstname>Maja</ns3:firstname>
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        <ns3:firstname>Žanka</ns3:firstname>
        <ns3:lastname>Bojić-Trbojević</ns3:lastname>
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        <ns3:firstname>Mirjana</ns3:firstname>
        <ns3:lastname>Nacka-Aleksić</ns3:lastname>
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        <ns3:title1>doktor medicinskih nauka-farmacija</ns3:title1>
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  <ns12:digitalbook>
    <ns12:name_magazine language="en">Small New World 2.0 Abstract Book, 4-5 September 2023., Graz, Austria</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles (SrbEVs) with Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), and Slovenian Network for Extracellular Vesicles (SiN-EV)</ns12:publisher>
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