All rights reserved Extracellular vesicles, human plasma info:eu-repo/semantics/conferenceProceedings 2024 https://phaidrabg.bg.ac.rs/o:36234 ISBN: 978-86-905626-1-9 Serbian Society for Extracellular Vesicles, SrbEVs with Austrian Society for Extracellular Vesicles, ASEV Baltic Society for Extracellular Vesicles, BSEV Italian Society for Extracellular Vesicles, EVIta French Society for Extracellular Vesicles, FSEV Spanish Society for Extracellular Vesicles, GEIVEX German Society for Extracellular Vesicles, GSEV United Kingdom Society for Extracellular Vesicles, UKEV ABSTRACT Introduction: Extracellular vesicles (EVs) have important roles in physiological and pathological conditions, thus could be used as promising biomarkers in clinical practice. There is a need for appropriate methods for EVs isolation that bypass major challenges of current methods such as obtaining low-yield and impurity of obtained vesicles. Therefore, it is crucial to establish methods for isolating that are economical, easy-to-operate and reproducible. The aim of this work was to utilize nanobodies, obtained from a naïve library by direct panning on EVs from human cell lines, for immunocapturing of exosomes from healthy-donor plasma. 2nd MOVE Symposium-Abstract Book-2024, Belgrade, Serbia eng application/pdf 1627721 bytes Implementation of Single-domain antibodies-based approach for isolation of extracellular vesicles from human plasma Tursunović, Marija Filipović, Lidija de Marco, Ario Kosanović, Maja Popović, Milica