
<ns0:uwmetadata xmlns:ns0="http://phaidra.univie.ac.at/XML/metadata/V1.0" xmlns:ns1="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0" xmlns:ns10="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0" xmlns:ns11="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0/entity" xmlns:ns12="http://phaidra.univie.ac.at/XML/metadata/digitalbook/V1.0" xmlns:ns13="http://phaidra.univie.ac.at/XML/metadata/etheses/V1.0" xmlns:ns2="http://phaidra.univie.ac.at/XML/metadata/extended/V1.0" xmlns:ns3="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/entity" xmlns:ns4="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/requirement" xmlns:ns5="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/educational" xmlns:ns6="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/annotation" xmlns:ns7="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/classification" xmlns:ns8="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/organization" xmlns:ns9="http://phaidra.univie.ac.at/XML/metadata/histkult/V1.0">
  <ns1:general>
    <ns1:identifier>o:36198</ns1:identifier>
    <ns1:title language="en">Arabidopsis thaliana root cells interact with outer membrane vesicles (OMVs) produced by plant beneficial bacterial strain Paraburkholderia phytofirmans PsJN </ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Introduction: Plant growth-promoting bacteria (PGPB) play an essential role in the growth and development of plants by facilitating the uptake of nutrients and increasing stress resistance. The interaction between plants and their beneficial bacteria is multifaceted, but the role of extracellular vesicles (EVs) in this interkingdom communication is poorly understood. To investigate the interaction of outer membrane vesicles (OMVs) with plant cells, EVs produced by Gram-negative bacteria, we isolated and characterized OMVs produced by Paraburkholderia phytofirmans PsJN, a PGPB strain known for its plant-protective abilities. Methods: The OMVs of P. phytofirmans PsJN were isolated using an affinity-based chromatography system and characterized by Nanoparticle tracking analysis (NTA). To monitor the interaction of OMVs with plant cells, the vesicles were labelled with the lipid-binding dye Vybrant™ DiD. To ensure the specificity of the signals, different methods to remove the unbound dye were tested, namely dialysis, ultracentrifugation, ultrafiltration and iodixanol density gradient purification. Arabidopsis thaliana roots were incubated with DiD-labelled OMVs and observed by confocal laser scanning microscopy (CLMS). Results: NTA revealed 70nm to 180 nm size range of isolated OMVs. </ns1:description>
    <ns1:keyword language="en">outer membrane vesicles (OMVs), Paraburkholderia phytofirmans PsJN</ns1:keyword>
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      <ns2:identifier>978-86-905626-1-9</ns2:identifier>
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    <ns1:upload_date>2025-05-23T12:43:26.118Z</ns1:upload_date>
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    <ns2:peer_reviewed>yes</ns2:peer_reviewed>
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        <ns3:firstname>Dragana</ns3:firstname>
        <ns3:lastname>Nikolić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Sofija</ns3:firstname>
        <ns3:lastname>Nešić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Aleksandra</ns3:firstname> 
       <ns3:lastname>Divac Rankov</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Dragana</ns3:firstname>
        <ns3:lastname>Bosnić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Vesna</ns3:firstname>
        <ns3:lastname>Spasovski</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Maja</ns3:firstname>
        <ns3:lastname>Kosanović</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
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        <ns3:firstname>Jelena</ns3:firstname>
        <ns3:lastname>Samardžić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, Belgrade, Serbia</ns3:institution>
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    <ns12:name_magazine language="en">2nd MOVE Symposium Abstract book, 8-11 October 2024, Belgrade, Serbia</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles, SrbEVs with Austrian Society for Extracellular Vesicles, ASEV Baltic Society for Extracellular Vesicles, BSEV Italian Society for Extracellular Vesicles, EVIta French Society for Extracellular Vesicles, FSEV Spanish Society for Extracellular Vesicles, GEIVEX German Society for Extracellular Vesicles, GSEV United Kingdom Society for Extracellular Vesicles, UKEV</ns12:publisher>
    <ns12:releaseyear>2024</ns12:releaseyear>
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