o:36179 Radical Scavenging and Ion-Reducing Capacity of Fumaria officinalis Extracts Obtained by Traditional and Assisted Extraction Techniques en The present research aimed to extract antioxidants from the fumitory aerial part in the flowering stage (containing leaves, stems, and flowers) by performing traditional and novel extraction procedures (maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)). The fumitory macerate showed significantly lower ABTS radical scavenging activity, expressed as a higher IC50 value (the concentration of extract required to neutralize 50% of radicals, 11.4 ± 0.1 mg/mL), in comparison to the other two extracts, whose IC50 values varied in a narrow range (8.6–9.5 mg/mL). In the DPPH assay, the trend was different: MAE (11.4 ± 0.3 mg/mL) ≥ UAE (12.0 ± 0.8 mg/mL) ≥ macerate (12.8 ± 0.1 mg/mL). In the CUPRAC assay, the UAE and MAE extracts (17.84 ± 0.85 and 18.05 ± 0.71 µmol Trolox equivalents (TE)/g, respectively) showed significantly higher antioxidant activity compared to the macerate (16.43 ± 0.45 µmol TE/g). Regarding the results of the FRAP method, there was no statistically significant difference in ferric ion reduction between the macerate, UAE, and MAE extracts (3.00–3.27 µmol Fe2+/g). However, the extract prepared using MAE provided the highest antioxidant potential, as shown in all four tests used. Due to demonstrated extracts’ antioxidant properties, additional research could address additional biological effects or the creation of delivery systems or encapsulates for the controlled delivery of fumitory bioactives. extraction; fumitory; antioxidant assays; polyphenols https://doi.org/10.3390/proceedings2025119002 2025-05-19T06:59:12.352Z 44 yes 46 Rabiea Ashowen Ahmoda University of Belgrade, Faculty of Technology and Metallurgy, Belgrade, Serbia Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Violeta Milutinović University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia person Dragana Dekanski University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicninskih nauka person 0000-0001-8467-4165 Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Belgrade, erbia person Aleksandra A. Jovanović Institute for the Application of Nuclear Energy INEP, University of Belgrade, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 219720 https://phaidrabg.bg.ac.rs/o:36179 no yes 16 70 92000004 11A29 11A41 Radical Scavenging and Ion-Reducing Capacity of Fumaria officinalis Extracts Obtained by Traditional and Assisted Extraction Techniques 2 119 1 Proceedings Basel, Switzerland MDPI 2025