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  <ns1:general>
    <ns1:identifier>o:36145</ns1:identifier>
    <ns1:title language="en">Extracellular vesicles from saliva of Rheumatoid Arthritis patients have a distinct profile compared to healthy controls</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Introduction: Rheumatoid arthritis (RA) is a systemic inflammatory disease that not only affects the joints but can also cause significant disability and impacts overall health, including oral health. Clinical studies have demonstrated that patients with RA often have worse oral health compared to healthy individuals. Saliva has been recognized as a valuable diagnostic tool for detecting both local and systemic diseases, including RA. However, using whole saliva presents several challenges, such as variability in composition and potential contamination. Therefore, salivary extracellular vesicles (EVs) have been investigated to overcome these barriers. The aim of this study was to isolate and  characterize salivary EVs from patients with RA in comparison to healthy controls. Methods: Salivary EVs were isolated from saliva pools of healthy volunteers and patients with RA by differential centrifugation, followed by characterization using SDS-PAGE, dot-blot, Western blot and NTA. Results: Analysis of the number, size, and zeta potential showed that the number and size of RA salivary EVs were greater than those isolated from healthy donors. Additionally, the zeta potential of RA salivary EVs was negative, unlike the zeta potential of salivary vesicles isolated from healthy volunteers. Within the overall similarity in protein composition and CD9- and CD63-immunoreactivity in the examined groups, a difference was observed in the presence of CD81, which was found only in RA salivary EVs. </ns1:description>
    <ns1:keyword language="en">Rheumatoid arthritis (RA), salivary extracellular vesicles </ns1:keyword>
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      <ns2:resource>1552100</ns2:resource>
      <ns2:identifier>978-86-905626-1-9</ns2:identifier>
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    <ns1:upload_date>2025-04-28T12:06:16.331Z</ns1:upload_date>
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    <ns2:peer_reviewed>yes</ns2:peer_reviewed>
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        <ns3:firstname>Marko</ns3:firstname>
        <ns3:lastname>Prokić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:orcid>0009-0005-1331-5372</ns3:orcid>
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        <ns3:firstname>Maja</ns3:firstname>
        <ns3:lastname>Kosanović</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
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 <ns3:firstname>Ivica</ns3:firstname>
        <ns3:lastname>Jeremić</ns3:lastname>
        <ns3:institution>Institute of Rheumatology, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Žanka</ns3:firstname>
        <ns3:lastname>Bojić-Trbojević</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
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        <ns3:orcid>0000-0001-5619-1027</ns3:orcid>
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    <ns1:size>1614763</ns1:size>
    <ns1:location>https://phaidrabg.bg.ac.rs/o:36145</ns1:location>
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    <ns1:cost>no</ns1:cost>
    <ns1:copyright>yes</ns1:copyright>
    <ns1:license>1</ns1:license>
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    <ns1:purpose>70</ns1:purpose>
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  <ns12:digitalbook>
    <ns12:name_magazine language="en">2nd MOVE Symposium-Abstract Book-2024, Belgrade, Serbia</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles, SrbEVs with Austrian Society for Extracellular Vesicles, ASEV Baltic Society for Extracellular Vesicles, BSEV Italian Society for Extracellular Vesicles, EVIta French Society for Extracellular Vesicles, FSEV Spanish Society for Extracellular Vesicles, GEIVEX German Society for Extracellular Vesicles, GSEV United Kingdom Society for Extracellular Vesicles, UKEV</ns12:publisher>
    <ns12:releaseyear>2024</ns12:releaseyear>
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