o:36140 Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts en Winter savory (Satureja montana L.) is a widely known medicinal plant species, which has been cultivated from seeds at the experimental field of the Institute “Dr Josif Pančić”, Pančevo, Serbia. The plant material used in this study was harvested from a 6-year-old plant. The aim of the study was to optimize the standard maceration process for polyphenol extraction from air-dried plant material, utilizing 50% ethyl alcohol solution. The optimization was performed through varying two most important parameters for maceration: solid-to-solvent ratio and extraction time. The obtained extracts were characterized via analyzing the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential (ABTS, DPPH, FRAP, and CUPRAC assays), extraction yield, conductivity, density, surface tension, and viscosity. The TPC varied from 7.15±0.8 to 92.2±2.5 mg gallic acid equivalent (GAE)/g of plant material. Further, the extraction time and solid-to-solvent ratio had significant influence on TFC; the highest values were achieved between 30 and 90 min, and at a 1:40 ratio. The highest ABTS●+ and DPPH● radical scavenging activities were detected in the extracts prepared using 1:30-1:50 ratios, while the best ferric and cupric ion reducing antioxidant potential was measured in the extract obtained at a 1:50 ratio. The highest extraction yield was detected in the extract obtained at a 1:50 ratio (86.0±2.9%), whereas the extraction time did not have a statistically significant influence on the mentioned parameter. On the other hand, the highest value of conductivity was measured in the extract prepared at a 1:10 ratio, and the highest density and surface tension of the extract was obtained at a ratio of 1:40 (0.941±0.002 g/mL and 29.0±0.1 mN/m, respectively), while the highest viscosity was measured in the extract prepared at a 1:20 ratio (2.89±0.02 mPa·s). In the present study, the optimization of the extraction was performed, in specific, the extraction time and solid-to-solvent ratio levels for improving the efficiency of polyphenols extraction from S. montana were determined. Our study shows the possibilities for production of polyphenols-rich extract of S. montana which might be used in pharmaceutical, food or cosmetic products. 1552100 978-86-904450-1-1 2025-04-24T12:14:28.692Z 44 yes 46 Natalija Čutović Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Tatjana Markovic Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Dragoja Radanovic Institute for Medicinal Plants Research “Dr Josif Pančić” Belgrade, Serbia person Branko Bugarski University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandar Marinkovic University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović Institute for the Application of Nuclear Energy INEP, University of Belgrade, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 333513 https://phaidrabg.bg.ac.rs/o:36140 no yes 19 70 92000004 11A29 11A2910 11A41 Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts O-2 Book of Abstract of the 1st Serbian Conference on Materials Application and Technology - SCOM Belgrade, Serbia Društvo za razvoj nauke Srbije 2022-10