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    <ns1:title language="en">Extracellular vesicles derived from mesenchymal stem/stromal cells derived from dental pulp of exfoliated teeth induce osteogenic differentiation</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
INTRODUCTION: Large bone defects in the field of orthopedic surgery remains challenging, primarily due to restricted regenerative capacity of bone tissue. The biomimetic bone implants could improve the osteoregeneration process, but the optimal composition for biomimetics remains unknown. Considering the great potential of extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) in regenerative medicine, our aim was to investigate the osteoinductive potential of EVs derived from MSCs from the dental pulp of human exfoliated deciduous teeth (SHEDs) either undifferentiated or those undergone differentiation into osteoblasts (osteoSHED-EVs), for potential use in biomimetic bone implants. EXPERIMENTAL: SHEDs were isolated from the dental pulp of 5 different donors of deciduous teeth and subjected to characterization by Flow cytometry (FCM). EVs-SHED and EVs-osteoSHED were isolated by sequential ultracentrifugation, and analysed by Western blot, NTA, and electron microscopy. Uptake of PKH67-labelled EVs by SHED was analysed by confocal microscopy and FCM. </ns1:description>
    <ns1:keyword language="en">Large bone defects; bone regeneration; extracellular vesicles; internalization</ns1:keyword>
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        <ns3:firstname>Marija</ns3:firstname>
        <ns3:lastname>Milivojević</ns3:lastname>
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        <ns3:firstname>Maja</ns3:firstname>
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        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
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        <ns3:firstname>Djordje</ns3:firstname>
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        <ns3:firstname>Sergej</ns3:firstname>
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    <ns12:name_magazine language="en">Chemical Industry-Supplementary Issue ExcellMater Conference 2024 Abstracts</ns12:name_magazine>
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