application/pdf 1814816 bytes Extracellular vesicles derived from mesenchymal stem/stromal cells derived from dental pulp of exfoliated teeth induce osteogenic differentiation https://phaidrabg.bg.ac.rs/o:36089 ISSN: 0367–598X http://creativecommons.org/licenses/by/4.0/legalcode ABSTRACT INTRODUCTION: Large bone defects in the field of orthopedic surgery remains challenging, primarily due to restricted regenerative capacity of bone tissue. The biomimetic bone implants could improve the osteoregeneration process, but the optimal composition for biomimetics remains unknown. Considering the great potential of extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) in regenerative medicine, our aim was to investigate the osteoinductive potential of EVs derived from MSCs from the dental pulp of human exfoliated deciduous teeth (SHEDs) either undifferentiated or those undergone differentiation into osteoblasts (osteoSHED-EVs), for potential use in biomimetic bone implants. EXPERIMENTAL: SHEDs were isolated from the dental pulp of 5 different donors of deciduous teeth and subjected to characterization by Flow cytometry (FCM). EVs-SHED and EVs-osteoSHED were isolated by sequential ultracentrifugation, and analysed by Western blot, NTA, and electron microscopy. Uptake of PKH67-labelled EVs by SHED was analysed by confocal microscopy and FCM. 2024 info:eu-repo/semantics/conferenceProceedings Milivojević, Marija Kosanović, Maja Bekić, Marina Čolić, Miodrag Janaćković, Djordje Tomić, Sergej eng Savez hemijskih inženjera Srbije Chemical Industry-Supplementary Issue ExcellMater Conference 2024 Abstracts volume: 78 startpage: 13 Large bone defects; bone regeneration; extracellular vesicles; internalization