
<ns0:uwmetadata xmlns:ns0="http://phaidra.univie.ac.at/XML/metadata/V1.0" xmlns:ns1="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0" xmlns:ns10="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0" xmlns:ns11="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0/entity" xmlns:ns12="http://phaidra.univie.ac.at/XML/metadata/digitalbook/V1.0" xmlns:ns13="http://phaidra.univie.ac.at/XML/metadata/etheses/V1.0" xmlns:ns2="http://phaidra.univie.ac.at/XML/metadata/extended/V1.0" xmlns:ns3="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/entity" xmlns:ns4="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/requirement" xmlns:ns5="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/educational" xmlns:ns6="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/annotation" xmlns:ns7="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/classification" xmlns:ns8="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/organization" xmlns:ns9="http://phaidra.univie.ac.at/XML/metadata/histkult/V1.0">
  <ns1:general>
    <ns1:identifier>o:36063</ns1:identifier>
    <ns1:title language="en">Influence of SEC to resistance of EVs to purity control treatments and stability in different handling/storage conditions</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Introduction: To utilize diagnostic/therapeutic potential of extracellular vesicles (EVs), their integrity should be kept throughout the separation, purification and storage process. However, some techniques, such as size exclusion chromatography (SEC), can affect the corona of EVs and influence both diagnostic/therapeutic molecules and stability of EVs. Therefore, the aim of this work was to investigate whether SEC affects EVs’ resistance to purity control treatments and stability in different handling/storage conditions. Materials &amp; Methods: We separated EVs by ultracentrifugation (UC) on 17000xg (T17) and 100.000xg (T100) and subjected half of obtained EVs to SEC (Sepharose 2B CL, 11 x 1.2 cm column). In each fraction, the number of particles was determined by NTA, protein concentration by BCA and EVs’ marker profile by dot-blot. Fractions containing peak of EVs were pooled. EVs before and after SEC were incubated with different detergents, buffers and were subjected to different temperatures after which the median size and concentration were determined by NTA. Tripsin was used for direct removal of corona. </ns1:description>
    <ns1:keyword language="en">extracellular vesicles (EVs), exclusion chromatography (SEC)</ns1:keyword>
    <ns2:identifiers>
      <ns2:resource>1552100</ns2:resource>
      <ns2:identifier>978-86-905626-0-2</ns2:identifier>
    </ns2:identifiers>
    <ns2:identifiers>
      <ns2:resource>1552102</ns2:resource>
      <ns2:identifier>https://srbevs.rs/small-new-world-2-0/</ns2:identifier>
    </ns2:identifiers>
  </ns1:general>
  <ns1:lifecycle>
    <ns1:upload_date>2025-04-04T12:36:23.267Z</ns1:upload_date>
    <ns1:status>44</ns1:status>
    <ns2:peer_reviewed>yes</ns2:peer_reviewed>
    <ns1:contribute seq="0">
      <ns1:role>46</ns1:role>
      <ns1:entity seq="0">
        <ns3:firstname>Sofija</ns3:firstname>
        <ns3:lastname>Glamočlija</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:orcid>0000-0003-0497-5852</ns3:orcid>
      </ns1:entity>
      <ns1:entity seq="4">
        <ns3:firstname>Marina</ns3:firstname>
        <ns3:lastname>Bekić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0002-6945-4241</ns3:orcid>
      </ns1:entity>
      <ns1:entity seq="3">
        <ns3:firstname>Alisa</ns3:firstname>
        <ns3:lastname>Gruden-Movsesijan</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0002-3061-5474</ns3:orcid>
      </ns1:entity>
      <ns1:entity seq="2">
        <ns3:firstname>Sergej</ns3:firstname>
        <ns3:lastname>Tomić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0003-2570-1295</ns3:orcid>
      </ns1:entity>
      <ns1:entity seq="1">
        <ns3:firstname>Maja</ns3:firstname>
        <ns3:lastname>Kosanović</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0002-1143-1805</ns3:orcid>
      </ns1:entity>
    </ns1:contribute>
  </ns1:lifecycle>
  <ns1:technical>
    <ns1:format>application/pdf</ns1:format>
    <ns1:size>231762</ns1:size>
    <ns1:location>https://phaidrabg.bg.ac.rs/o:36063</ns1:location>
  </ns1:technical>
  <ns1:rights>
    <ns1:cost>no</ns1:cost>
    <ns1:copyright>yes</ns1:copyright>
    <ns1:license>1</ns1:license>
  </ns1:rights>
  <ns1:classification>
    <ns1:purpose>70</ns1:purpose>
  </ns1:classification>
  <ns1:organization>
    <ns8:hoschtyp>92000004</ns8:hoschtyp>
    <ns8:orgassignment>
      <ns8:faculty>11A29</ns8:faculty>
      <ns8:department>11A2904</ns8:department>
    </ns8:orgassignment>
  </ns1:organization>
  <ns12:digitalbook>
    <ns12:name_magazine language="en">Small New World 2.0-Abstract Book, September 2023</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles (SrbEVs) with Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), and Slovenian Network for Extracellular Vesicles (SiN-EV)</ns12:publisher>
    <ns12:releaseyear>2023</ns12:releaseyear>
  </ns12:digitalbook>
</ns0:uwmetadata>