Milivojević, Marija Kosanović, Maja Tomić, Sergej Bekić, Marina Čolić, Miodrag Janaćković, Djordje 2024 Collagen Hydrogel Embedded SHED-EVs for Enhanced Osteogenesis ABSTRACT Introduction: Mesenchymal stem/stromal cells (MSCs) from the dental pulp of human exfoliated deciduous teeth (SHED cells) have shown promising results in bone tissue regeneration. The application of SHED-derived extracellular vesicles (EVs) to bone defects can be achieved using biomaterial scaffolds. Collagen scaffolds, which are natural polymers with slow degradation times, are particularly suitable for EVs entrapment. This study aimed to examine the release rate of embedded SHED-EVs from collagen scaffolds and to evaluate their osteogenic capacity when combined with collagen and gradually released. Methods: SHED-EVs were isolated using differential ultracentrifugation and characterized using nanotracking particle analysis (NTA), Western Blot (WB), and scanning electron microscopy (SEM). All rights reserved eng Mesenchymal stem/stromal cells (MSCs), SHED-derived extracellular vesicles (EVs) https://phaidrabg.bg.ac.rs/o:36057 ISBN: 978-86-905626-1-9 application/pdf 1607790 bytes info:eu-repo/semantics/conferenceProceedings 2nd MOVE Symposium Abstract book, 8-11 October 2024, Belgrade, Serbia Serbian Society for Extracellular Vesicles, SrbEVs with Austrian Society for Extracellular Vesicles, ASEV Baltic Society for Extracellular Vesicles, BSEV Italian Society for Extracellular Vesicles, EVIta French Society for Extracellular Vesicles, FSEV Spanish Society for Extracellular Vesicles, GEIVEX German Society for Extracellular Vesicles, GSEV United Kingdom Society for Extracellular Vesicles, UKEV