2024 Milivojević, Marija Janaćković, Djordje Čolić, Miodrag Bekić, Marina Tomić, Sergej Kosanović, Maja Visualization of Extracellular Vesicles Derived from Dental Mesenchymal Stem/Stromal Cells Using SEM and Immunogold TEM Analyses All rights reserved ABSTRACT Extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have emerged as promising therapeutic agents in regenerative medicine. EVs are nanosized, membrane-limited vesicles containing protein and RNA cargo, released by various cell types for intercellular communication. It has been shown that EVs derived from MSCs play a significant role in the tissue regeneration process [1]. Dental MSCs such as human stem cells from the dental pulp of deciduous teeth (SHED cells) are easily accessible MSCs with high proliferative capacity [2]. The objective of this study was to isolate and characterize EVs derived from SHED, to explore their potential utility in regenerative medicine applications. SHED cells were isolated from deciduous teeth from three donors, and their morphology and osteogenic potential were assessed through optical microscopy and Alizarin Red staining of cells cultured in osteogenic cell media for 21 days. eng Extracellular vesicles (EVs), mesenchymal stem/stromal cells (MSCs) https://phaidrabg.bg.ac.rs/o:36055 ISBN: 978-86-6184-056-2 application/pdf 1016402 bytes Third International Conference On Electron Microscopy Of Nanostructures, ELMINA 2024, Program and Book of Abstracts info:eu-repo/semantics/conferenceProceedings Serbian Academy of Sciences and Arts, Belgrade