o:36054 Investigating the effect of extracellular vesicles from Trichinella spiralis on reduction of respiratory allergy induced by ovalbumin in BALB/c mice en ABSTRACT Introduction: We have recently discovered that extracellular vesicles from Trichinella spiralis muscle larvae (TsEVs) exhibit immunomodulatory properties in vitro. Our goal was to investigate the potential of TsEVs to ameliorate ovalbumin (OVA)-induced allergy in a murine model. Material & Methods: Experimental allergic airway inflammation was induced in BALB/c mice. Treatment by intranasal administration of TsEVs was performed on the days of sensitization and challenge. Cells from bronchoalveolar lavage, lungs and speen were stained and analyzed by FACS or cultivated for cytokine analysis. Results: TsEVs treatment led to a significant reduction in the OVA-specific IgE in the sera, decrease in number of eosinophils in the bronchoalveolar lavage and decline in percentage of eosinophils, macrophages and NK cells in the lungs of treated animals. Trichinella spiralis muscle larvae (TsEVs), BALB/c mice, respiratory allergy 1552100 978-80-11-05123-5 2025-04-01T07:37:41.340Z 44 yes 46 Maja Kosanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka 0000-0002-1143-1805 Sofija Glamočlija University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia person 0000-0003-0497-5852 Anna Schmid Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria person Nataša Radulović Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia person Alisa Gruden-Movsesijan University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor bioloških nauka person 0000-0002-3061-5474 Ljiljana Sabljić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia person 0000-0001-6027-591X Saša Vasilev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Belgrade, Serbia doktor medicinskih nauka-veterina person 0000-0001-7870-2713 Irma Schabussova Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria person application/pdf 692492 https://phaidrabg.bg.ac.rs/o:36054 no yes 1 70 92000004 11A29 11A2904 11A21 ASEV-CzeSEV joint meeting on Extracellular Vesicles-Proceedings CzeSEV, z.s. Brno 2024