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    <ns1:title language="en">Isolation of extracellular vesicles from resistant tumor cells using nanobodies-based immunoaffinity approach</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Introduction: Extracellular vesicles (EVs) are an important contributing factor to drug resistance in cancer. In order to study their features and elucidate their molecular composition. To that extent, we have decided to use two pairs of multi-drug resistant (MDR) cancer cell lines (non-small cell lung carcinoma NCI-H460/R and glioblastoma U87-TxR) and their sensitive counterparts (H460 and U87, respectively ion and contribution in drug resistance, EVs need to isolated in an efficient manner and sufficient quantities. Broadening new possibility-ties in EV-based diagnostics requires innovative, adaptable, and affordable methods for the scalable isolation of high-purity EVs from different sources. This study aims to adapt high-performance immune capture chromatography based on nanobody technology for EVs isolation from cell culture media of MDR cancer cells and their sensitive cells. </ns1:description>
    <ns1:keyword language="en">Extracellular vesicles (EVs), nanobodies </ns1:keyword>
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      <ns2:identifier>978-86-905626-1-9</ns2:identifier>
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      <ns2:resource>1552102</ns2:resource>
      <ns2:identifier>https://srbevs.rs/2nd-move-symposium/</ns2:identifier>
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    <ns1:upload_date>2025-04-01T07:05:51.100Z</ns1:upload_date>
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        <ns3:firstname>Lidija</ns3:firstname>
        <ns3:lastname>Filipović</ns3:lastname>
        <ns3:institution>Innovative Centre of the Faculty of Chemistry, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Milica</ns3:firstname>
        <ns3:lastname>Popović</ns3:lastname>
        <ns3:institution>University of Belgrade, Faculty of Chemistry, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Milica</ns3:firstname>
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        <ns3:institution>Institute for Biological Research &quot;Siniša Stanković&quot;, National Institute of the Republic of Serbia, Belgrade, Serbia</ns3:institution>
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        <ns3:institution>Institute for Biological Research &quot;Siniša Stanković&quot;, National Institute of the Republic of Serbia, Belgrade, Serbia</ns3:institution>
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<ns3:firstname>Marija</ns3:firstname>
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        <ns3:institution>Institute for Biological Research &quot;Siniša Stanković&quot;, National Institute of the Republic of Serbia, Belgrade, Serbia</ns3:institution>
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        <ns3:title1>doktor bioloških nauka</ns3:title1>
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    <ns12:name_magazine language="en">2nd MOVE Symposium Abstract book, 8-11 October 2024, Belgrade, Serbia</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles SrbEVs with Austrian Society for Extracellular Vesicles ASEV, Baltic Society for Extracellular Vesicles BSEV, Italian Society for Extracellular Vesicles EVIta, French Society for Extracellular Vesicles FSEV, Spanish Society for Extracellular Vesicles GEIVEX, German Society for Extracellular Vesicles GSEV, United Kingdom Society for Extracellular Vesicles UKEV</ns12:publisher>
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