o:36031 The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis en ABSTRACT Background: Ferroptosis is one of the processes that could drive immune-mediated neurodegeneration in multiple sclerosis (MS). Exosomes as biologically active extracellular nanovesicles can carry miRNAs and are easily delivered across blood - brain barrier. Our aim is to provide data about the difference in network interplay of exosome carried miRNAs and PBMC mRNA between mild and severe MS phenotypes, in context of ferroptosis process regulation. Methods: Targeted mRNA sequencing of selected ferroptosis related genes was performed in PBMC of two MS phenotypes on Illumina iSeq100 NGS instrument. DESeq2 algorithm was used for obtaining DE genes and miRNA/mRNA interplay was woven using miRNET platform. multiple sclerosis, mRNA, ferroptosis 1552100 978-86-905626-0-2 1552102 https://srbevs.rs/small-new-world-2-0/ 2025-03-20T13:20:18.469Z 44 yes 46 Jovana Kuveljić "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia Ivan Jovanović "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia person Maja Kosanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1143-1805 Nataša Mačak "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia person Tamara Djurić "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia person Aleksandra Stanković "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia person Maja Živković "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Laboratory for radiobiology and molecular genetics, Belgrade, Serbia person application/pdf 232771 https://phaidrabg.bg.ac.rs/o:36031 no yes 1 70 92000004 11A28 11A2810 11A29 11A2904 Small New World 2.0-Abstract Book, Graz, Austria 2023 Serbian Society for Extracellular Vesicles (SrbEVs) with Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), and Slovenian Network for Extracellular Vesicles (SiN-EV) 2023