o:36025 Fumaria officinalis L. Extracts: Cytotoxic, Antioxidant, and Anti-Inflammatory Potential in Human Keratinocytes en Fumaria officinalis L. (fumitory, Fumariaceae) is recognized as a valuable source of natural bioactives and is widely used for its diverse pharmacological properties. This study aimed to examine the beneficial effects of fumitory extracts related to skin. Specifically, extracts’ cytotoxicity, antioxidant and anti-inflammatory properties, as well as sun protection factor (SPF) were examined. Cytotoxicity was evaluated in HaCaT human keratinocyte cells using a concentration range of 25 to 100 μg/mL, identifying all tested concentrations as non-cytotoxic. The production of intracellular reactive oxygen species (ROS) in HaCaT cells, exposed to the extract with or without hydrogen peroxide treatment, was measured using the H2DCFDA assay (25-100 μg/mL). The results demonstrated that fumitory extracts showed significant in vitro antioxidant capacity. The anti-inflammatory potential of the extracts, i.e., the impact of the extracts on pro-inflammatory cytokines: interleukin-1β (IL-1β) and macrophage inhibitory factor (MIF) was also evaluated using cell-based ELISA (100 μg/mL). Therefore, it was shown that in bacterial lipopolysaccharide (LPS)-treated cells, fumitory extracts significantly reduced IL-1β and MIF expression in comparison to LPS alone, confirming the anti inflammatory activity of the extracts against LPS challenge. Nevertheless, the SPF values of fumitory extracts at tested concentrations (25-100 μg/mL) were low (0.29-1.25). Regarding the results related to antioxidant and anti-inflammatory properties, findings suggest that fumitory extracts represent a promising source of bioactives with potential application in pharmaceutical, cosmetic, or dermo cosmetic formulations. 2025-03-18T09:38:04.001Z 44 yes 46 Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor tehnoloških nauka 0000-0001-5394-0125 Rabiea Ahmoda Faculty of Technology and Metallurgy University of Belgrade person Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Violeta Milutinović Faculty of Pharmacy, University of Belgrade person Dragana Dekanski University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor medicinskih nauka person 0000-0001-8467-4165 Aleksandar Marinković Faculty of Technology and Metallurgy University of Belgrade person application/pdf 279816 https://phaidrabg.bg.ac.rs/o:36025 no yes 19 70 92000005 11A29 11A41 Fumaria officinalis L. Extracts: Cytotoxic, Antioxidant, and Anti-Inflammatory Potential in Human Keratinocytes 5 Proceedings of International Conference on Dermatology and Cosmetology Conference (ICDECOCO-25) 5 Proceedings of International Conference on Dermatology and Cosmetology Conference (ICDECOCO-25) International Society for Applied Research 2025