http://creativecommons.org/licenses/by/4.0/legalcode MDPI, Basel, Switzerland https://phaidrabg.bg.ac.rs/o:35964 doi:10.3390/molecules30050993 eng molecules volume: 30 number: 5 biotherapeutics; isolation and purification; ion-exchange; IgG; transferrin Simultaneous Isolation and Purification of Transferrin and Immunoglobulin G from Human Serum-A New Biotech Solution info:eu-repo/semantics/article application/pdf 3624315 bytes ABSTRACT A fast and simple biotech method is presented for the simultaneous isolation and purification of transferrin (Tf) and immunoglobulin G (IgG) from the same pool-sample of human serum, yielding >98% pure proteins. Serum sample preparation was achieved by precipitation with ethacridine lactate (rivanol). Protein purification was performed with AKTA Avant 150 FPLC, using a Resource Q column. Three different buffers at pH 6.2 (MES, phosphate, and Bis-Tris) were tested. Isolated and purified proteins retained their native 3D structure, as shown by spectrofluorimetric measurements. Tf functionality was preserved, as confirmed by the retention of both the iron binding capacity and its ability to interact with the transferrin receptor (immunofluorescent staining), as well as the immunogenicity of IgG, as shown by Western blot analysis with immunodetection. The formation of IgG aggregates was avoided. This biotech method is a rapid, simple, and time-saving alternative to other methods for the isolation of extremely pure IgG and Tf, while it is also the only method so far described for their simultaneous isolation. 2025 Četić, Danilo Miljuš, Goran Dobrijević, Zorana Gligorijević, Nikola Vilotić, Aleksandra Nedić, Olgica Penezić, Ana