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    <ns1:title language="en">Isolation of extracellular vesicles from resistant tumor cells using nanobodies-based immunoaffinity approach</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Introduction: Extracellular vesicles (EVs) are an important contributing factor to drug resistance in cancer. In order to study their features and elucidate their molecular composition. To that extent, we have decided to use two pairs of multi-drug resistant (MDR) cancer cell lines (non-small cell lung carcinoma NCI-H460/R and glioblastoma U87-TxR) and their sensi- tive counterparts (H460 and U87, respectively ion and contribution in drug resistance, EVs need to isolated in an efficient manner and sufficient quantitives. Broadening new possibili- ties in EV-based diagnostics requires innovative, adaptable, and affordable methods for the scalable isolation of high-purity EVs from different sources. This study aims to adapt high-performance immune capture chromatography based on nanobody technology for EVs isolation from cell culture media of MDR cancer cells and their sensitive cells.</ns1:description>
    <ns1:keyword language="en">MDR; extracellular vesicles; nanobody technology</ns1:keyword>
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      <ns2:resource>1552100</ns2:resource>
      <ns2:identifier>978-86-905626-1-9</ns2:identifier>
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    <ns1:upload_date>2025-02-20T09:49:01.040Z</ns1:upload_date>
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        <ns3:firstname>Lidija</ns3:firstname>
        <ns3:lastname>Filipović</ns3:lastname>
        <ns3:institution>Innovative Centre of the Faculty of Chemistry, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Marija</ns3:firstname>
        <ns3:lastname>Tursunović</ns3:lastname>
        <ns3:institution>Innovative Centre of the Faculty of Chemistry, Belgrade, Serbia</ns3:institution>
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        <ns3:firstname>Maja</ns3:firstname>
        <ns3:lastname>Kosanović</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor bioloških nauka</ns3:title1>
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        <ns3:firstname>Marija</ns3:firstname>
        <ns3:lastname>Grozdanić</ns3:lastname>
        <ns3:institution>Institute for Biological Research &quot;Siniša Stanković&quot;, National Institute of the Republic of Serbia, University of Belgrade</ns3:institution>
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        <ns3:institution>Institute for Biological Research &quot;Siniša Stanković&quot;, National Institute of the Republic of Serbia, University of Belgrade</ns3:institution>
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        <ns3:firstname>Milica</ns3:firstname>
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    <ns12:name_magazine language="en">Abstract book: 2nd MOVE Symposium; 2024 Nov 8-11; Belgrade, Serbia</ns12:name_magazine>
    <ns12:publisher>Serbian Society for Extracellular Vesicles, SrbEVs; Austrian Society for Extracellular Vesicles, ASEV; Baltic Society for Extracellular Vesicles, BSEV; Italian Society for Extracellular Vesicles, EVIta; French Society for Extracellular Vesicles, FSEV; Spanish Society for Extracellular Vesicles, GEIVEX; German Society for Extracellular Vesicles, GSEV; United Kingdom Society for Extracellular Vesicles, UKEV</ns12:publisher>
    <ns12:releaseyear>2024</ns12:releaseyear>
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