o:35758 Fumaria officinalis dust as a source of bioactives for potential dermal application: optimization of extraction procedures, phytochemical profiling, and effects related to skin health benefits en Fumaria officinalis (fumitory), in the form of dust, was employed as a source of bioactive extracts whose chemical profile and biological potential were investigated. According to the results of the optimization of the extraction protocol, the extract with the highest polyphenol yield was prepared using fumitory dust under the optimal conditions determined using the statistical tool, 23 full factorial design: 50% ethanol and a 30:1 mL/g ratio during 120 s of microwave extraction (22.56 mg gallic acid equivalent/g of plant material). LC-MS and spectrophotometric/gravimetric analyses quantified the polyphenol, flavonoid, tannin, alkaloid, and protein contents. Caffeoylmalic acid, quercetin dihexoside, quercetin pentoside hexoside, rutin, and methylquercetin dihexoside were the most dominant compounds. The highest total flavonoid, condensed tannin, alkaloid, and protein yields were determined in the extract prepared using microwaves. In addition to the proven antioxidant potential, in the present study, the anti-inflammatory activity of fumitory extracts is also proven in the keratinocyte model, as well as a significant reduction of H2O2-induced reactive oxygen species production in cells and the absence of keratinocyte cytotoxicity. Thus, detailed chemical profiles and investigated biological effects related to skin health benefits encourage the potential application of fumitory dust extracts in dermo-cosmetic and pharmaceutical preparations for dermatological circumstances. alkaloids; bioactives; extraction; Fumaria officinalis; optimization; plant dust; polyphenols 1552099 https://doi.org/10.3390/plants14030352 2025-01-24T13:01:49.772Z 44 yes 46 Rabiea Ahmoda University of Belgrade Faculty of Technology and Metallurgy Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Violeta Milutinović University of Belgrade, Faculty of Pharmacy, Serbia person Milena Milošević Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade Belgrade, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović Institute for the Application of Nuclear Energy INEP, University of Belgrade, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 1581831 https://phaidrabg.bg.ac.rs/o:35758 no yes 16 70 92000001 11A41 11A13 11A29 11A24 Fumaria officinalis dust as a source of bioactives for potential dermal application: optimization of extraction procedures, phytochemical profiling, and effects related to skin health benefits 14 14 3 352 Plants Basel, Switzerland MDPI 2025