o:35365 Polyphenol and protein content in wild thyme dust extracts with natural deep eutectic solvents en Natural deep eutectic solvents (NADESs) are a green and nontoxic alternative to potentially toxic organic extraction mediums. Wild thyme (Thymus serpyllum L.) contains a wide range of bioactive compounds that have an important role in its antioxidant, antimicrobial, carminative, expectorant, analgesic, stimulant, diaphoretic, antispasmodic, diuretic, and anti-inflammatory effects. The aim of this study was to evaluate wild thyme dust in terms of polyphenol and protein content. Wild thyme dust was from the Institute for Medicinal Plants Research “Dr Josif Pančić”, Pančevo, Serbia. The extraction was performed at room temperature in the incubator shaker KS 4000i control (IKA, Germany) using four different NADESs (citric acid/glucose, citric acid/saccharose, malic acid/glucose, and malic acid/ sacharose with 50% of water) and a solvent-to-solid ratio of 30:1 mL/g for 75 min. Total polyphenol and protein content were measured employing spectrophotometric methods. Polyphenol concentration varied from 28.6 to 45.1 mg gallic acid equivalent/g of plant material, while protein content was in a range of 21.4 to 25.5 mg of albumin equivalent/g of plant material. In both measured parameters, the efficiency of the employed NADESs follows the trend: citric acid/saccharose≥citric acid/glucose>malic acid/sacharose≥malic acid/glucose. Wild thyme dust extract prepared using citric acid/saccharose in comparison to other tested NADESs was favored as a potential ingredient in foods, functional foods, dietetic supplements, or pharmaceuticals. dust, NADES, polyphenol, protein, Thymus serpyllum L. 1552101 3044-2117 2024-12-16T22:11:48.502Z 44 yes 46 Aleksandra Jovanović Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia doktor tehnoloskih nauka 0000-0001-5394-0125 Nemanja Krgović Institute for Medicinal Plants Research “Dr. Josif Pančić“, Belgrade, Serbia person Ana Alimpić Aradski University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade, Serbia person Jelena Živković Institute for Medicinal Plant Research “Dr. Josif Pančić”, Serbia, Belgrade person Katarina Šavikin Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person application/pdf 418735 https://phaidrabg.bg.ac.rs/o:35365 no yes 19 70 92000004 11A29 11A2910 11A02 Polyphenol and protein content in wild thyme dust extracts with natural deep eutectic solvents 111 Book of Abstracts of Natural Resources, Green Technology, and Sustainable Development Conference Pierottijeva 6, 10000 Zagreb, Croatia University of Zagreb, Faculty of Food Technology and Biotechnology 2024