o:35361 Advancing Immunoassay Precision: A Novel Preanalytical Method for Enhancing Thyroglobulin Measurement in the Presence of Tg Antibodies Unapredjenje preciznosti imunoeseja: Nova preanalitička metoda za poboljšanje merenja tiroglobulina u prisustvu Tg antitela en ABSTRACT Thyroglobulin (Tg) is a reliable marker for detecting recurrence in differentiated thyroid cancer (DTC) patients, but frequently occurring Tg antibodies (TgAbs) can hinder accurate measurement. We aimed to develop a preanalytical protocol for precise Tg detection in TgAb presence using the immunoradiometric assay (IRMA) platform. This study involved forty-five patients who underwent IRMA Tg and radioimmunoassay (RIA) TgAb measurements, including two patients monitored for recurrence and one with confirmed recurrence. All three had undetectable Tg levels. We evaluated three preanalytical methods in aiming to separate Tg from TgAbs: buffer only (Protocol 1), micro-spin filters only (Protocol 2), and a combination of both (Protocol 3). Tg; Tg immunoassays; IRMA; preanalytical treatment; TgAb interference; DTC follow-up; recurrent DTC 1552099 10.3390/ijms252413252 2024-12-16T07:35:29.155Z 44 yes 46 Ilona Djorić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka 0000-0001-5322-4559 Aleksandra Todorović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0003-2225-8988 Marija Gnjatović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biotehničkih nauka person 0000-0001-8233-1636 Snežana Golubović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia diplomirani farmaceut, magistar nauka-imunologija person 0000-0002-4551-4342 Miloš Žarković Clinic for Endocrinology, Diabetes and Metabolic Diseases, Clinical Centre of Serbia, Belgrade, Serbia person Jelena Janković Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2589-3306 Tijana Išić Denčić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8475-2266 Sonja Šelemetjev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-2898-6671 application/pdf 1311455 https://phaidrabg.bg.ac.rs/o:35361 no yes 16 70 92000001 11A29 International Journal of Molecular Sciences 25 24 MDPI, Basel, Switzerland 2024