o:35281 Cytotoxic, Antioxidant, and Anti-Genotoxic Properties of Combretastatin A4 in Human Peripheral Blood Mononuclear Cells: A Comprehensive In Vitro Study en ABSTRACT Despite significant advances in drug discovery and the promising antitumor potential of combretastatin A4 (CA-4), which selectively targets rapidly dividing cancer cells, CA-4’s effects on non-dividing human cells, such as peripheral blood mononuclear cells (PBMCs), remain unclear. The aim of this study is to evaluate the in vitro bioactivity of CA-4 in human PBMCs, focusing on its antigenotoxic and antioxidant properties, while comparing its cytotoxic potency against PBMCs, cancer cell lines (JAR and HeLa), and the normal trophoblast cell line HTR 8/SVneo. Cell viability and metabolic activity were evaluated using the MTT assay. combretastatin A4; PBMC; oxidative stress; genotoxicity; trophoblast 1552099 10.3390/biom14121535 2024-12-03T10:46:24.039Z 44 yes 46 Petar Popović Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Serbia Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Dijana Topalović Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Serbia person Lada Živković Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Serbia person Milica Marković Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Serbia person Biljana Spremo-Potparević Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Serbia person application/pdf 1353393 https://phaidrabg.bg.ac.rs/o:35281 no yes 16 70 92000001 11A13 11A29 11A2901 Biomolecules 14 1535 MDPI, Basel, Switzerland 2024