o:35261 Skin beneficial effects of pomegranate peel extract and its dominant compounds en Introduction: The peel of pomegranate (Punica granatum L., Punicaceae) fruit is usually treated as a waste. On the other hand, it is a rich source of polyphenols, including phenolic acids, flavonoids, and tannins. A broad range of biological activities was reported for pomegranate peel extracts. Material and Methods: Extract of pomegranate peel (PPE) was obtained by double percolation using 50% EtOH and spray dried. HPLC was applied for the chemical analysis of the most abundant phenolics. PPE and individual compounds were tested using in vitro inhibition of hyaluronidase, elastase, collagenase, and tyrosinase. In vitro anti-inflammatory potential of PPE was investigated in the erythrocyte membrane stabilization assay. Results: The most abundant compounds in PPE was punicalagin, followed by punicalin, ellagic and gallic acid. PPE showed IC50 values of 534.76 µg/ml for hyaluronidase, 651.81 µg/ml for elastase, 1013.81 µg/ml for collagenase, and 537.60 µg/ml for tyrosinase inhibition. The most active individual compound was ellagic acid, except for collagenase inhibition assay where punicalin was the strongest inhibitor (IC50 value of 95.63 µg/ml). Also, PP extract showed promising concentration-dependent in vitro anti-inflammatory activity. Conclusion: Both, PPE and individual compounds showed promising skin beneficial effects to meet trends in cosmetic and healthcare sector 1552099 10.60738/03ak-mn82 2024-11-29T20:09:53.396Z 44 yes 46 Katarina Šavikin Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Koscuska 1, Serbia, Belgrade Ana Alimpić Aradski University of Belgrade-Faculty of Biology, Institute of Botany and Botanical Garden „Jevremovac“, Serbia person Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy, Belgrade, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 Nada Ćujić Nikolić Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Koscuska 1, Serbia, Belgrade person Jelena Živković Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Koscuska 1, Serbia, Belgrade person application/pdf 677364 https://phaidrabg.bg.ac.rs/o:35261 no yes 19 70 92000005 11A02 11A29 Skin beneficial effects of pomegranate peel extract and its dominant compounds 129 Book of Abstracts of the 17 th World Congress on Polyphenols Applications Italy University of Milan 2024