o:35217 Association between genetic variants in HSA-MIR-27A and HSA-MIR-146A genes and male infertility Asocijacija varijanti u genima HSA-MIR-27A i HSA-MIR-146A sa muškim sterilitetom en ABSTRACT Background: miRNAs have enormous potential to be used as diagnostic and prognostic markers as well as therapeutic targets in male infertility and diseases of the reproductive system. This study aimed to investigate the association between the two functional genetic variants in the hsa-miR27a (rs2910164) and hsa-miR-146a gene (rs895819) and male infertility in North Macedonian population, as well as to test their association with the values of major seminal parameters. Methods: The case group included in this study comprised 158 men initially diagnosed with idiopathic male infertility. The control group included 126 age-matched healthy male volunteers who fathered at least one child. Results: We report the association of rs2910164 minor allele C for the first time with the increased susceptibility to asthenoteratozoospermia. Additionally, our results indicating the association of allele C with low sperm vitality are a novel finding. We did not demonstrate the association between genetic variant rs895819 and the risk of different types of male infertility. Still, the number of participants with CC genotype in subjects diagnosed with asthenoteratozoospermia was null, while in controls, it reached 7.2%. We further detected the rs895819 genotype-dependent difference in rapid progressive sperm motility. male infertility, rs2910164, rs895819, polymorphism, miR-27a, miR-146a 1552099 10.5937/jomb0-50876 1552101 1452-8258 2024-11-25T09:33:55.102Z 44 yes 46 Srećko Rajovski Private Healthcare Institution Clinical Hospital "Acibadem Sistina", Skopje, North Macedonia Nikoleta Milanović University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person Nemanja Radovanović University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person Miloš Brkušanin University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person Dušanka Savić-Pavićević University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 Goran Brajušković University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person Suzana Matijašević Joković University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia person application/pdf 265437 https://phaidrabg.bg.ac.rs/o:35217 no yes 16 70 92000001 11A02 11A29 11A2905 Journal of Medical Biochemistry 43 6 936 945 2024