application/pdf 265437 bytes https://phaidrabg.bg.ac.rs/o:35217 doi:10.5937/jomb0-50876 ISSN: 1452-8258 2024 eng Journal of Medical Biochemistry volume: 43 number: 6 startpage: 936 endpage: 945 male infertility, rs2910164, rs895819, polymorphism, miR-27a, miR-146a http://creativecommons.org/licenses/by/4.0/legalcode info:eu-repo/semantics/article Rajovski, Srećko Milanović, Nikoleta Radovanović, Nemanja Brkušanin, Miloš Savić-Pavićević, Dušanka Dobrijević, Zorana Brajušković, Goran Matijašević Joković, Suzana ABSTRACT Background: miRNAs have enormous potential to be used as diagnostic and prognostic markers as well as therapeutic targets in male infertility and diseases of the reproductive system. This study aimed to investigate the association between the two functional genetic variants in the hsa-miR27a (rs2910164) and hsa-miR-146a gene (rs895819) and male infertility in North Macedonian population, as well as to test their association with the values of major seminal parameters. Methods: The case group included in this study comprised 158 men initially diagnosed with idiopathic male infertility. The control group included 126 age-matched healthy male volunteers who fathered at least one child. Results: We report the association of rs2910164 minor allele C for the first time with the increased susceptibility to asthenoteratozoospermia. Additionally, our results indicating the association of allele C with low sperm vitality are a novel finding. We did not demonstrate the association between genetic variant rs895819 and the risk of different types of male infertility. Still, the number of participants with CC genotype in subjects diagnosed with asthenoteratozoospermia was null, while in controls, it reached 7.2%. We further detected the rs895819 genotype-dependent difference in rapid progressive sperm motility. Association between genetic variants in HSA-MIR-27A and HSA-MIR-146A genes and male infertility