application/pdf 2101358 bytes Bioactivity of methanolic pulp extract from pumpkin (Cucurbita moschata) in human keratinocytes https://phaidrabg.bg.ac.rs/o:35098 cobiss:155106313 ISBN: 978-86-7994-063-6 ABSTRACT The study investigated the potential of pumpkin pulp extracts from two Cucurbita moschata accessions grown in Serbia as skin-friendly agents with antioxidant and sun protecting properties. The research aimed to address the underrepresentation of pumpkin pulp in bioactivity studies despite its potential as a rich source of beneficial compounds for skin health. Methanolic pumpkin pulp extracts (MPE) were prepared from two accessions, MO 31 and MO 32, sourced from the breeding collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. The MPE were characterized for their carotenoid content using HPLC-DAD, revealing different levels of carotenoids in each accession. Cumulative carotenoid content in MO 31 and MO 32 was 0.29 and 0.09 mg/g, respectively. The cytotoxicity of the extracts was assessed using viability assay MTT in human keratinocyte (HaCaT) cells, and showed no significant decrease in cell viability at concentrations of 10, 100, and 1000 μg/mL after 24 hours of exposure. 2024 info:eu-repo/semantics/conferenceProceedings eng pumpkin extract, keratinocytes, skin, bioactivity, reactive oxygen species, cytotoxicity, photoprotective http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode Pirković, Andrea Krstić, Sanja Jovanović, Aleksandra Dekanski, Dragana Miljić, Milorad University of Novi Sad, Institute of Food Technology in Novi Sad 5th International Congress Food Technology, Quality and Safety, e-Abstract Book