o:35097 Exploring health beneficial effects of poisonous mushroom Paxillus involutus Batsch Fr. Ispitivanje korisnih efekata otrovne pečurke Paxillus involutus Batsch Fr. en ABSTRACT In the present study, phenolic and flavonoid composition and biological properties of methanolic extract of wild growing Paxillus involutus collected in Serbia have been investigated. Ellagic acid was the most abundant phenolic compound (34.92 µg g-1), followed by 5-O-caffeoylquinic acid (4.51 µg g-1), whereas isoorientin was the most abundant flavonoid (3.42 µg g-1). P. involutus turned out to be a rich source of phenolic compounds (74.67 mg GAE g-1 d.w.), whereas total flavonoid content was significantly lower (4.05 mg QE g-1 d.w.). As for the various investigated biological activities, methanolic extract exerted high level of antioxidant, antimicrobial and antibiofilm activities. The highest antioxidative potential was measured by TAC (350 TE mg g-1 d.w.), whereas evaluation of antimicrobial properties showed selective antimicrobial potential toward tested pathogenic microorganisms, with resistant strain of E. coli being the most susceptible to the activity of the extract (MIC 0.08 mg mL-1, MBC 0.16 mg mL-1). Furthermore, methanolic extract of P. involutus demonstrated genotoxicity, severe hemolysis effects and selective cytotoxicity against colon cancer cells. From the obtained data, it may be concluded that investigated mushroom albeit being toxic for human consumption, may be considered as a source of highly bioactive components with potential application in drug development. Chemical characterization, antioxidant, genotoxicity, cytotoxicity, antimicrobial potential 1552099 10.1080/01480545.2024.2412776 1552101 1525-6014 2024-11-08T09:46:34.509Z 44 yes 46 Jovana Petrović Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-6423-4645 Uroš Gašić Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia person Biljana Nikolić Faculty of Biology, University of Belgrade, Belgrade, Serbia person Stefana Cvetković Faculty of Biology, University of Belgrade, Belgrade, Serbia person Jasmina Glamočlija Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia person application/pdf 62653 https://phaidrabg.bg.ac.rs/o:35097 no yes 18 70 92000001 11A21 11A02 11A29 11A2905 Drug and Chemical Toxicology Taylor & Francis Inc. 2024