o:3492 Izolacija i karakterizacija bioaktivnih sekundarnih metabolita iz odabranih sojeva roda Streptomyces doktorska disertacija Isolation and characterization of bioactive secondary metabolites from selected Streptomyces strains : doctoral dissertation sr Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR. Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR. Biologija - Primenjena mkrobiologija / Biology - Applied microbiology Datum odbrane : 6.12.2012 bioaktivna jedinjenja, sekundarni metaboliti, Streptomyces, pigmenti, undecilprodigiozin, izoalkanske masne kiseline, biciklični peptidi, Streptomyces durmitorensis, PKS. bioactive compounds, secondary metabolites, Streptomyces, pigments, undecylprodigiosine, iso-branched fatty acids, bicyclic peptides, Streptomyces durmitotensis, PKS yes 1024553906 91552101 87 91552100 1024553906 2013-02-01T10:07:00.053Z 45 no 46 mentor Nada N. Stanković 2012 63 mentor Branka Vasiljević 2012 63 mentor Đorđe Fira 2012 63 član komisije Branka Vasiljević 2012 63 član komisije Đorđe Fira 2012 63 član komisije Jasmina Nikodinović-Runić 2012 PDF/A (179 listova) 6414656 http://phaidrabg.bg.ac.rs/o:3492 no yes 4 2013-02-01T10:07:00.320Z 70 11 1066841 1066882 Biologija - Primenjena mkrobiologija / Biology - Applied microbiology Datum odbrane : 6.12.2012. bioaktivna jedinjenja, sekundarni metaboliti, Streptomyces, pigmenti, undecilprodigiozin, izoalkanske masne kiseline, biciklični peptidi, Streptomyces durmitorensis, PKS bioactive compounds, secondary metabolites, Streptomyces, pigments, undecylprodigiosine, iso-branched fatty acids, bicyclic peptides, Streptomyces durmitotensis, PKS [579.873.7 : 579.222] (043.3) Bioaktivna jedinjenja 1738 11A02 2012